OGS106
PSF-CMV-PUC19 - CMV PUC19 MCS PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
UNSPSC Code:
12352200
Recommended Products
form
buffered aqueous solution
mol wt
size 4309 bp
bacteria selection
ampicillin
Origin of replication
pUC (500 copies)
Peptide cleavage
no cleavage
Promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
reporter gene
none
shipped in
ambient
storage temp.
−20°C
General description
A versatile cloning plasmid for the expression of genes in mammalian cells. This plasmid contains the multiple cloning site (MCS) from pUC19 however it has been modified slightly to accommodate some restriction sites in our vector system. These changes are described in the full plasmid details PDF. The use of this MCS instead of the our normal vector MCS will limit the ability to use some of the inserts that we sell that immediately flank or are inserted within the standard MCS. This primarily includes N-terminal tags and signal peptides. This is because these inserts are flanked by restriction sites that are not compatible with the pUC19 MCS. Most other inserts should still be compatible with this plasmid.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
Application
Multiple cloning site notes: This vector contains a different multiple cloning site (MCS) from our standard vectors. The MCS is derived from pUC19. The order of the restriction sites in this vector are almost the same as in pUC19 however the sequence is not. The pUC19 MCS has also been modified as follows.
- The XbaI site in the pUC19 MCS has been replaced with a SpeI site (these sites produce compatible cohesive ends when cleaved).
- The XbaI site in the pUC19 MCS has been replaced with a SpeI site (these sites produce compatible cohesive ends when cleaved).
- The SbfI and PstI sites have been replaced with an NsiI site (which produces compatible cohesive ends with SbfI and PstI when cleaved).
- The BamHI site that is normally found in all SnapFast vectors (in the downstream fusion MCS) has been replaced with a BclI site because the pUC19 MCS contains this site. BamHI and BclI produce compatible cohesive ends when cleaved although BclI is methylated in this vector and will require growth in a Dam methylase negative bacterial strain (such as JM110 cells) before it can be used.
- The ClaI to NheI sites have other functions such as adding peptide tags or IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site.
Sequence
To view sequence information for this product, please visit the product page
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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