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1.46000

Millipore

HEIMPLATE Violet Red Bile Dextrose Agar Settle plate

90 mm settle plate with 30 ml filling volume for detection and enumeration of Enterobacteriaceae and bile-tolerant Gram-negative bacteria from pharmaceutical and other food products

Synonym(s):

VRBD, Violet Red Bile Glucose agar, Crystal Violet Neutral Red Bile Glucose agar

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About This Item

UNSPSC Code:
41106217
NACRES:
NA.74

Agency

APHA
EP
ISO 21528
JP
USP

Quality Level

sterility

sterile

form

plate (ready-to-use)

feature

ready-to-use

packaging

pkg of (20 or 120 plates per box)

technique(s)

microbiological culture: suitable

pH

7.4 ( in H2O)

application(s)

microbiology
pharmaceutical

storage temp.

15-25°C

suitability

Escherichia coli
Salmonella spp.
coliforms

Related Categories

General description

Violet Red Bile Dextrose (VRBD) Agar settle plates are used for the detection and enumeration of Enterobacteriaceae and bile-tolerant Gram-negative bacteria. Crystal violet and bile salts inhibit the accompanying bacterial flora. Fermentation of glucose by Enterobacteriaceae leads to acid production, which is indicated by a color change to red and by zones of precipitated bile acids surrounding the colonies. Enzymatic digest of animal tissue provides carbon and nitrogen sources for the growth, and yeast extract primarily supplies the B-complex vitamins. The culture medium is not specific for Enterobacteriaceae as some other accompanying bacteria (e.g. Aeromonas) also show the same reaction.

Application

Violet Red Bile Dextrose (VRBD) Agar settle plates are used to detect and enumerate Enterobacteriaceae from food, animal feed, and other materials. They are also suitable for the enumeration of bile-tolerant Gram-negative bacteria from pharmaceuticals and other materials.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2


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Articles

Learn more about our culture media portfolio for microbial examination of non-sterile products, fully compliant with the harmonized Pharmacopoeia.

Protocols

The International Organization for Standardization (ISO) published the revised EN ISO 21528-1 and EN ISO 21528-2 standards in 2017.

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