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Merck
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Key Documents

06-755

Sigma-Aldrich

Anti-Histone H3.1t (H3-4) Antibody

UPSTATE®, rabbit polyclonal

Synonym(s):

Histone H3.1t, H3/t, H3t, H3/g

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥6,218.64

Available to ship onApril 27, 2025Details

Sign In for Best Price
Request a Bulk Order

Product Name

Anti-Histone H3 Antibody, 0.5 mg/mL, Upstate®

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, human

manufacturer/tradename

Upstate®

concentration

0.5 mg/mL

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particle size

5 μm

particle size

3 μm

particle size

3 μm

particle size

3 μm

matrix

silica gel, high purity, spherical base material, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

separation technique

hydrophilic interaction (HILIC), reversed phase, normal phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

cyano phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

pore size

180 Å

pore size

120 Å

pore size

120 Å

pore size

120 Å

General description

Histone H3, also known as Histone H3.1t, H3/t, H3t, or H3/g, and encoded by the gene HIST3H3/H3Ft, is one of the core components of the nucleosome. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The octamer wraps approximately 147 bp of DNA. A classic nuclear marker, new expression is seen during S phase of the cell cycle. Histone H3 is often acetylated and or methylated multiple times. Changes in methylation or acetylation state of histone H3 together with similar modification on the sister histones is one key aspect of epigenetic gene expression and regulation.

Specificity

Broad species cross-reactivity is expected based on sequence similarity.
This antibody recognizes Histone H3 at the N-terminus.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3.

Application

Anti-Histone H3 Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 also known as histone 3-H3 & has been validated in ChIP, ICC & WB.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in HeLa cells.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Histone H3 from 500 µg of HeLa acid extract. Immunoprecipitated sample was then subjected to Western Blotting using 0.5 µg/mL of the same antibody.

Chromatin Immunoprecipitaton: A representative lot from an independent laboratory immunoprecipitated Histone H3 from plant chromatin (Ng, D. W., et al. (2006). Plant Cell. 18(1):119-132.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 in 10 µg of HeLa acid extract.

Target description

~17 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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