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Merck
CN

07-449

Anti-trimethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Synonym(s):

H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3k27me3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

dry ice

target post-translational modification

trimethylation (Lys27)

Gene Information

human ... HIST1H3F(8968)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.

Application

Anti-trimethyl-Histone H3 (Lys27), also known as Anti-H3K27me3, is a highly published Rabbit Polyclonal Antibody. This protein A purified antibody is dot blot tested for trimethylated lysine 27 specificity and validated in WB, ICC, IP.
Immunocytochemistry:

Immunoprecipitation:

Beadlyte Histone-Peptide Specificity Assay:

Biochem/physiol Actions

Broad species cross-reactivity expected.
Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.

Physical form

Format: Purified
Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.

Analysis Note

Routinely evaluated by western blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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An extended transcriptional network for pluripotency of embryonic stem cells.
Kim, J; Chu, J; Shen, X; Wang, J; Orkin, SH
Cell null
A WD40 domain cyclophilin interacts with histone H3 and functions in gene repression and organogenesis in Arabidopsis.
Li, H; He, Z; Lu, G; Lee, SC; Alonso, J; Ecker, JR; Luan, S
Plant Cell null
Su(z)2 antagonizes auto-repression of Myc in Drosophila, increasing Myc levels and subsequent trans-activation.
Khan, A; Shover, W; Goodliffe, JM
Testing null
COOH-terminal binding protein regulates expression of the p16INK4A tumor suppressor and senescence in primary human cells.
Mroz, EA; Baird, AH; Michaud, WA; Rocco, JW
Cancer Research null
Polycomb-group proteins repress the floral activator AGL19 in the FLC-independent vernalization pathway.
Schonrock, N; Bouveret, R; Leroy, O; Borghi, L; Kohler, C; Gruissem, W; Hennig, L
Genes & Development null
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