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569255-U

Discovery^® HS C18 (3 µm) HPLC Columns

Name: Discovery<SUP>&#174;</SUP> HS C18 (3 µm) HPLC Columns
Brand: SUPELCO

L × I.D. 15 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:

41115700

eCl@ss:

32110501

NACRES:

SB.52

product name

Discovery^® HS C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

product line

Discovery^®

feature

endcapped

manufacturer/tradename

Discovery^®

packaging

1 ea of

extent of labeling

20% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 2.1 mm

surface area

300 m²/g

surface coverage

3.2 μmol/m²

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

3 μm

pore size

120 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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HPLC Columns

General description

Discovery^® HS C18 HPLC column (L × I.D. 15 cm × 2.1 mm) is found to be stable under acidic and basic conditions.

Features and Benefits

Stable, low bleed for LC-MS applications
Scalable from analytical to preparatory
Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility
Higher hydrophobicity for better resolution of difficult analytes

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Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

guard cartridge

Product No.

Description

Pricing

569277-U

Discovery^® HS C18 Supelguard^™ Guard Cartridge, Kit, 3 μm particle size, L × I.D. 2 cm × 2.1 mm

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Method development and validation for simultaneous determination of lumefantrine and its major metabolite, desbutyl lumefantrine in human plasma using RP-HPLC/UV detection.

Fazli Khuda et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 944, 114-122 (2013-12-10)

A simple, specific, precise and rapid RP-HPLC-UV method was developed for simultaneous determination of lumefantrine and its metabolite desbutyl lumefantrine in human plasma. Experimental parameters were optimized and the method was validated according to standard guidelines. The method showed adequate

Determination of lipoic acid in human plasma by HPLC-ECD using liquid-liquid and solid-phase extraction: Method development, validation and optimization of experimental parameters.

Abad Khan et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(28), 2782-2788 (2010-09-11)

A rapid, inexpensive, sensitive and specific HPLC-ECD method for the determination of lipoic acid in human plasma was developed and validated over the linearity range of 0.001-10μg/ml using naproxen sodium as an internal standard (IS). Extraction of lipoic acid and

Comparison of chaotropic salt and ionic liquid as mobile phase additives in reversed-phase high-performance liquid chromatography of biogenic amines.

Jolanta Flieger et al.

Journal of separation science, 34(7), 733-739 (2011-03-02)

Highly hydrophilic compounds belonging to biogenic amines were analysed in the reversed-phase system, modified with the addition of ionic liquids: 1-ethyl-3-methyl-imidazolium hexafluorophosphate (EMIM PF(6)) and chaotropic salt NaPF(6) on Discovery HS C18 column at acidic conditions. The effect of the

Simultaneous LC-MS-MS Determination of Lopinavir and Rifabutin in Human Plasma.

Swati Jaiswal et al.

Journal of chromatographic science, 55(6), 617-624 (2017-03-24)

Tuberculosis (TB) with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome represents the most common infectious diseases worldwide. Anti-TB drugs are used concurrently with antiretroviral drug for treatment of TB-HIV co-morbidities. Due to lower risk of interaction with protease inhibitors, rifabutin is

High-throughput LC-MS/MS assay for 6-methoxy-2-naphthylacetic acid, an active metabolite of nabumetone in human plasma and its application to bioequivalence study.

Bhavin N Patel et al.

Biomedical chromatography : BMC, 22(11), 1213-1224 (2008-07-25)

A simple, precise and accurate assay for the determination of 6-methoxy-2-naphthylacetic acid (6-MNA), an active metabolite of nabumetone in human plasma, was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analyte (6-MNA) and propranolol (internal standard, IS) were

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Protocols

HPLC Analysis of Polyphenols in Nero d'Avola Red Wine on Discovery® HS C18 (UV 280 nm)