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230162

Sigma-Aldrich

Sulforhodamine B

Dye content 75 %

Synonym(s):

Acid Red 52, Sulforhodamine B monosodium salt

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10 G
¥1,130.45
50 G
¥4,438.31
100 G
¥6,412.96
500 G
¥22,101.03

¥1,130.45

Available to ship on2025年4月24日Details

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10 G
¥1,130.45
50 G
¥4,438.31
100 G
¥6,412.96
500 G
¥22,101.03

About This Item

Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
3520-42-1
Molecular Weight:
580.65
Colour Index Number:
45100
Beilstein:
3886008
EC Number:
222-529-8
MDL number:
MFCD00010180
UNSPSC Code:
12171500
PubChem Substance ID:
24853778
NACRES:
NA.47

¥1,130.45

Available to ship on2025年4月24日Details

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Assay

≥73% (HPLC)

Quality Level

100

form

powder

composition

Dye content, 75%

technique(s)

microbe id | staining: suitable

color

brown to very dark brown
 green to very dark green

solubility

methanol: 1 mg/mL

λmax

554 nm

ε (extinction coefficient)

≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

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Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

solubility

water: 0.1 g/mL, colorless to faintly yellow

solubility

water: 20 mg/mL, clear, colorless to faintly yellow

solubility

-

solubility

water: 100 mg/mL at 25 °C, colorless to faintly yellow

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

optical activity

[α]/D 199°

optical activity

[α]/D 199°

optical activity

[α]/D 199°

optical activity

[α]20/D 190 to 201 °, c = 2% (w/v) in water

General description

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.[1]

Application

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.[2]

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000421134
    0000421134
    0000345171
    0000345171
    MKCF8839

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    Yuwei Chen et al.
    Bioscience reports, 40(5) (2020-05-05)
    The liver is a vital organ and the hepatic lobule serves as the most basic structural and functional unit which is mainly assembled with parenchymal cells including hepatocytes and biliary epithelial cells. The continuous tubular arrangement of biliary cells which
    Endocrine disrupting effects in vitro of conazole antifungals used as pesticides and pharmaceuticals
    Kj?rstad MB
    Reproductive Toxicology, 30, 573-582 (2010)
    Sulforhodamine B colorimetric assay for cytotoxicity screening
    Vichai V
    Nature Protocols, 1, 1112?1116-1112?1116 (2006)
    Christian A Ruge et al.
    Colloids and surfaces. B, Biointerfaces, 139, 219-227 (2016-01-01)
    The conversion of colloidal drug carriers/polymeric nanoparticles into dry microparticulate powders (e.g., by spray-drying) is a prominent approach to overcome the aerodynamic limitations of these formulations for delivery via inhalation. However, to what extent such nano-embedded microparticles disintegrate into individual/intact
    Anna Jaśkiewicz et al.
    Journal of clinical medicine, 8(5) (2019-05-19)
    The present study is centered on molecular mechanisms of the cytoprotective effect of geranylgeraniol (GGOH) in skeletal muscle harmed by statin-associated myopathy (SAM). GGOH via autophagy induction was purportedly assumed to prevent skeletal muscle viability impaired by statins, atorvastatin (ATR)
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