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Safety Information

OGS3099

Sigma-Aldrich

PSF-OXB20-NH2-OMPA - OMPA SECRETION PLASMID

plasmid vector for molecular cloning

Synonym(s):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

recombinant

expressed in E. coli

form

buffered aqueous solution

mol wt

size 3911 bp

bacteria selection

kanamycin

Origin of replication

pUC (500 copies)

Peptide cleavage

no cleavage

Peptide tag location

N-terminal

Promoter

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

reporter gene

none

Secretion signal

OmpA

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PSF-OXB20-NH2-OMPA - OMPA SECRETION PLASMID plasmid vector for molecular cloning

OGS3099

PSF-OXB20-NH2-OMPA - OMPA SECRETION PLASMID

PSF-OXB20-NH2-OMPT - OMPT SECRETION PLASMID plasmid vector for molecular cloning

OGS3161

PSF-OXB20-NH2-OMPT - OMPT SECRETION PLASMID

PSF-OXB20-NH2-OMPC - OMPC SECRETION PLASMID plasmid vector for molecular cloning

OGS3160

PSF-OXB20-NH2-OMPC - OMPC SECRETION PLASMID

PSF-OXB20-NH2-OMPA-FLAG®-EKT - OMPA SECRETION AND FLAG® TAG PLASMID plasmid vector for molecular cloning

OGS3067

PSF-OXB20-NH2-OMPA-FLAG®-EKT - OMPA SECRETION AND FLAG® TAG PLASMID

peptide tag location

N-terminal

peptide tag location

N-terminal

peptide tag location

N-terminal

peptide tag location

N-terminal

bacteria selection

kanamycin

bacteria selection

kanamycin

bacteria selection

kanamycin

bacteria selection

kanamycin

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

EKT

promoter

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

promoter

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

promoter

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

promoter

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

General description

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an Outer Membrane Protein A (OmpA) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.

Promoter Expression Level: PSF-OXB20-NH2-OMPA - OMPA secretion plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

OXB20-NH2-OMPA - OMPA secretion plasmid is an N-terminal OmpA secretory signal peptide plasmid. This bacterial expression vector has no protease or other tags.

Sequence

To view sequence information for this product, please visit the product page

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Geoffrey M Lynn et al.
    Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
    The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
    Diana Romero et al.
    Carcinogenesis, 37(1), 18-29 (2015-10-28)
    Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
    Jin-Gyoung Jung et al.
    PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
    The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
    Alexander C Cerny et al.
    PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
    Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual

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