Anti-NeuN Antibody (rabbit)

RNA binding protein fox-1 homolog 3 also known as NeuN (Neuronal nuclei) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID 52897) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Originally characterized as a marker of post-mitotic neurons and named neuronal nuclei (NeuN), Rbfox3 plays a role in promoting neuronal differentiation through alternative splicing of Numb pre-mRNA during brain development. Rbfox3-knockout in mice is reported to result in defective hippocampal gene expression, defects in synaptic transmission and plasticity in the dentate gyrus, as well as increased seizure susceptibility and decreased anxiety-related behaviors. Likewise, human RBFOX3 gene mutations have been linked to neurodevelopmental delay, cognitive impairments, autistic features, and epilepsy.

Anti NeuN antibody from rabbit is a polyclonal antibody that targets NeuN N-terminal region that ends at the start of the RNA recognition motif (RRM). The target region is present in all human, mouse, and rat spliced isoforms reported by UniProt (A6NFN3, Q8BIF2, D4A2H6, A0A0G2K2J4).

Epitope: N-terminus

GST-tagged recombinant mouse NeuN N-terrminal fragment.

Anti-NeuN (rabbit) Antibody, Cat. No. ABN78, is a highly specific rabbit polyclonal antibody that targets NeuN and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in cultured embryonic E18 rat hippocampus neurons.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN-positive neurons in human (cerebellum and cerebral cortex) and mouse (hippocampus) brain tissue sections.

Immunocytochemistry Analysis: A representative lots immunostained 4% paraformaldehyde-fixed neurons isolated from snail brain (Nikolić, L., et al. (2013). J. Exp. Biol. 216(Pt 18):3531-3541).

Immunofluorescence Analysis: Representative lots detected NeuN-positive neurons in frozen brain sections from 4% paraformaldehyde-perfused mice (Cherry, J.D., et al. (2015). J. Neuroinflammation. 12:203; Huang, B., et al. (2015). Neuron. 85(6):1212-1226; Ataka, K., et al. (2013). PLoS One. 8(11):e81744).

Immunofluorescence Analysis: A representative lots detected NeuN-positive neurons in formalin-fixed and paraffin-embedded amyotrophic lateral sclerosis (ALS) human brain tissue sections (Fratta, P., et al. (2015). Neurobiol. Aging. 36(1):546.e1-7).

Immunohistochemistry Analysis: A representative lot detected NeuN-positive neurons in rat brain tissue sections (Mendonça, M.C., et al. (2013).Toxins. 5(12):2572-2588).

Western Blotting Analysis: Representative lot detected NeuN in mouse and rat brain tissue lysates (Huang, B., et al. (2015). Neuron. 85(6):1212-1226; Mendonça, M.C., et al. (2013).Toxins. 5(12):2572-2588).

This rabbit polyclonal antibody is also avaiable as Alexa Fluor^™ 488 (ABN78A4), biotin (ABN78B), and Cy3 (ABN78C3) conjugates for immunocytochemistry, immunofluorescence, immunohistochemistry applications.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in mouse brain nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.

~48/42 kDa observed. 33.87/35.12 kDa (human isoform 1/2), 40.61/39.05/35.64/34.07/35.63 kDa (mouse isoform 1/2/3/4/5), 40.59kDa (rat; UniProt D4A2H6), 34.05 kDa (rat; UniProt A0A0G2K2J4) calculated. Uncharacterized bands may be observed in some lysate(s).

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse brain nuclear extract

Concentration: Please refer to lot specific datasheet.

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