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OGS1120

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PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

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Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
UNSPSC Code:
12352200
NACRES:
NA.85

tag

10-His tagged

form

buffered aqueous solution

mol wt

size 6201 bp

bacteria selection

kanamycin

mammalian cells selection

puromycin

Origin of replication

pUC (500 copies)

Peptide cleavage

TEV

Peptide tag location

C-terminal

Promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

reporter gene

none

shipped in

ambient

storage temp.

−20°C

General description

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Sequence

To view sequence information for this product, please visit the product page

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Articles

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

Sequencing Primers

Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.

DNA Component Explanation

Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.

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