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P9743

Sigma-Aldrich

pT7-FLAG-4 Expression Vector

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54 EA
¥1,713.42

¥1,713.42


Available to ship on2025年4月27日Details



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54 EA
¥1,713.42

About This Item

UNSPSC Code:
41106610
NACRES:
NA.32

¥1,713.42


Available to ship on2025年4月27日Details


shipped in

dry ice

Quality Level

storage temp.

−20°C

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This Item
570925712857094
matrix active group

carbon phase

matrix active group

carbon phase

matrix active group

carbon phase

matrix active group

carbon phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

particle size

120-400 mesh

particle size

120-400 mesh

particle size

120-400 mesh

particle size

120-400 mesh

application(s)

food and beverages

application(s)

food and beverages

application(s)

food and beverages

application(s)

food and beverages

material

polypropylene tube

material

polypropylene tube

material

polypropylene tube

material

polypropylene tube

volume

3 mL

volume

6 mL

volume

12 mL

volume

6 mL

Application

pT7-FLAGa-4 is a 6451 bp Escherichia coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAGÒ fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter [1][2] drives transcription of ORF-FLAG fusion constructs.

Legal Information

pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

Regulatory Information

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    A H Rosenberg et al.
    Gene, 56(1), 125-135 (1987-01-01)
    Plasmid vectors are described that allow cloning of target DNAs at sites where they will be minimally transcribed by Escherichia coli RNA polymerase but selectively and actively transcribed by T7 RNA polymerase, in vitro or in E. coli cells. Transcription
    F W Studier et al.
    Journal of molecular biology, 189(1), 113-130 (1986-05-05)
    A gene expression system based on bacteriophage T7 RNA polymerase has been developed. T7 RNA polymerase is highly selective for its own promoters, which do not occur naturally in Escherichia coli. A relatively small amount of T7 RNA polymerase provided

    Articles

    Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.

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