NA1020
GenElute™ PCR Clean-Up Kit
sufficient for 70 purifications
Synonym(s):
Gen Elute, PCR Purification
About This Item
Recommended Products
usage
sufficient for 70 purifications
technique(s)
DNA purification: suitable
storage temp.
15-25°C
Related Categories
1 of 4
This Item | PHR1126 | 76177 | 90115 |
|---|---|---|---|
| technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 100 |
| grade analytical standard | grade certified reference material, pharmaceutical secondary standard | grade certified reference material, TraceCERT® | grade analytical standard |
| shelf life limited shelf life, expiry date on the label | shelf life - | shelf life limited shelf life, expiry date on the label | shelf life limited shelf life, expiry date on the label |
| product line VETRANAL® | product line - | product line TraceCERT® | product line - |
General description
DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Application
Features and Benefits
- Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Removes over 99% of primers and other components
- Eliminates the need to remove mineral oil by organic extraction
- 40% more purification preps supplied than market leader
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Purifies up to 100 μL or 10 μg of PCR amplified DNA in 8 minutes
- Removes over 99% of primers and other components
Other Notes
Principle
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Can Product NA1020, GenElute™ PCR Clean-Up Kit, be used for isolation of PCR products labeled with digoxigenin?
Although we have not tested the kit with digoxigenin-labeled PCR products specifically, it should be fine to use
Can Product No. NA1020, GenElute™ PCR Clean-Up Kit, be used to clean up PCR products larger than 10 kb?
We haven't tested anything bigger than 10 kb, but we would expect larger DNA to bind well. That said, the bound DNA might be more difficult to elute. Heating the elution solution to 60-65°C may help with elution. Incubating a few minutes to allow the elution solution to soak into the binding matrix may also help.
When using Product No. NA1020, GenElute™ PCR Clean-Up Kit, what is the largest amount of PCR reaction sample that can be processed on one column?
The upper limit on the PCR reaction volume that can be cleaned up is 100 μl. In that scenario, 500 μl of binding solution would be added for a total of 600 μl volume being applied to the column.
When using GenElute™ PCR Clean-Up Kit, Product No. NA1020, Can the amount of elution buffer be reduced in order to obtain a more concentrated eluate?
One may indeed increase concentration by reducing elution volume, but we would not suggest going lower than 25-30 μl. One must keep in mind that if a volume of less than 50 μl is used (for example, 30 μl), the concentration will increase, but the total yield will also be reduced.
Can GenElute™ PCR Clean-Up Kit, Product NA1020, be used for isolation of labeled PCR products?
This kit will work with labeled PCR products and no modifications to the standard protocol are needed.
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