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Key Documents

Safety Information

D1947

Sigma-Aldrich

Diffinity RapidTip®

for PCR Purification

Synonym(s):

PCR clean up tip, PCR purification tip, PCR reaction clean-up tip

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About This Item

EC Number:
UNSPSC Code:
41106300
NACRES:
NA.52

manufacturer/tradename:
(Diffinity Genomics, Inc.)
Pricing and availability is not currently available.

Quality Level

form

solid

manufacturer/tradename

(Diffinity Genomics, Inc.)

capacity

50(µL)

storage temp.

room temp

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1 of 4

This Item
DTTROD9779D0632
DTT OmniPur® Grade, >= 99.4%, Used for reduction of protein disulfide linkages

3860OP

DTT

DTT crystalline powder, =97% (Ellman′s reagent), Mr 154.3

DTTRO

DTT

solubility

water: 5%

solubility

-

solubility

H2O: 50 mg/mL

solubility

H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

Quality Level

100

Quality Level

-

Quality Level

200

Quality Level

200

form

crystalline powder

form

crystalline powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Diffinity RapidTip effectively removes dNTPs, primers and primer dimers while providing greater than 90% recovery of pure DNA fragments from 100 bp to 10 kb. The functional pipette tip contains everything you need for PCR purification. The tip is filled with a proprietary adsorption technology that has a differential affinity for PCR components. The dispensed solution yields purified, high quality DNA ready for downstream applications such as DNA sequencing, SNP analysis and microarray printing.

Application

Diffinity RapidTip® has been used in PCR product purification.[1]

Features and Benefits

  • Single step
  • Recovers 90% of high quality dsDNA
  • Optimized for 25 μL RXN

Legal Information

RapidTip is a registered trademark of Diffinity Genomics, Inc.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

STOT RE 2 Inhalation

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    C N Roterman et al.
    Proceedings. Biological sciences, 280(1764), 20130718-20130718 (2013-06-21)
    The phylogeny of the superfamily Chirostyloidea (Decapoda: Anomura) has been poorly understood owing to limited taxon sampling and discordance between different genes. We present a nine-gene dataset across 15 chirostyloids, including all known yeti crabs (Kiwaidae), to improve the resolution
    Myron T La Duc et al.
    Applied and environmental microbiology, 78(16), 5912-5922 (2012-06-26)
    Spacecraft hardware and assembly cleanroom surfaces (233 m(2) in total) were sampled, total genomic DNA was extracted, hypervariable regions of the 16S rRNA gene (bacteria and archaea) and ribosomal internal transcribed spacer (ITS) region (fungi) were subjected to 454 tag-encoded
    Lisa A Santry et al.
    Virus research, 175(1), 30-44 (2013-04-16)
    Maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV) are related members of a group of small ruminant lentiviruses (SRLVs) that infect sheep and goats. SRLVs are endemic in many countries, including Canada. However, very little is known about the
    Daniel D Rhoads et al.
    International journal of molecular sciences, 13(3), 2535-2550 (2012-04-11)
    Clinical diagnostics of chronic polymicrobial infections, such as those found in chronic wounds, represent a diagnostic challenge for both culture and molecular methods. In the current retrospective study, the results of aerobic bacterial cultures and culture-free bacterial identification using DNA
    Jason W Sahl et al.
    Applied and environmental microbiology, 78(14), 4884-4892 (2012-05-15)
    The sequencing and analysis of multiple housekeeping genes has been routinely used to phylogenetically compare closely related bacterial isolates. Recent studies using whole-genome alignment (WGA) and phylogenetics from >100 Escherichia coli genomes has demonstrated that tree topologies from WGA and

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