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A4685

Sigma-Aldrich

Luminol sodium salt

luminescent, ≥98% (titration), powder and chunks

Synonym(s):

3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione

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1 EA
¥16,094.23

¥16,094.23

Available to ship on2025年4月27日Details

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1 EA
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About This Item

Linear Formula:
C8H6N3O2Na
CAS Number:
20666-12-0
Molecular Weight:
199.14
MDL number:
MFCD00161491
UNSPSC Code:
12352204
PubChem Substance ID:
24890898
NACRES:
NA.83

¥16,094.23

Available to ship on2025年4月27日Details

Product Name

Luminol sodium salt,

Assay

≥98% (titration)

Quality Level

300

form

powder and chunks

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless to faint red

SMILES string

O=C1NN([Na])C(C2=CC=CC(N)=C21)=O

InChI

1S/C8H7N3O2.Na/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12;/h1-3H,(H4,9,10,11,12,13);/q;+1/p-1

InChI key

RVJVDCVIJCBUTH-UHFFFAOYSA-M

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59246U

SUPELCOGEL™ 8H, 8% Crosslinked HPLC Column

separation technique

ion exclusion

separation technique

ligand exchange

separation technique

ion exclusion

separation technique

ion exclusion

particle size

9 μm

particle size

9 μm

particle size

9 μm

particle size

9 μm

matrix

sulfonated polystyrene/divinylbenzene, spherical particle platform, polymeric

matrix

sulfonated polystyrene/divinylbenzene, spherical particle platform, polymeric

matrix

sulfonated polystyrene/divinylbenzene, spherical particle platform, polymeric

matrix

sulfonated polystyrene/divinylbenzene, spherical, polymeric

product line

SUPELCOGEL™

product line

SUPELCOGEL™

product line

SUPELCOGEL™

product line

SUPELCOGEL™

agency

suitable for USP L17

agency

suitable for USP L19

agency

suitable for USP L17

agency

suitable for USP L17

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

Application

Luminol sodium salt has been used:

  • as a component of MEBSS buffer in a bioluminescence assay to detect peroxidase activity in whole blood samples[1]
  • to study protein interaction in whole-cell lysates by immunoprecipitation[2]
  • for in vitro reactive oxygen species (ROS) quantification in CD45+ and CD45− cells[3]

Biochem/physiol Actions

Luminol is a redox-sensitive chemiluminescent compound.[4][5] It emits blue luminescence when the basic aqueous solution of luminol having oxygen atoms come in contact with oxidizing agents like potassium ferricyanide.[4] It is highly stable and is applicable in various fields such as analytical chemistry, metallurgy, biochemistry, and forensics for detecting reactive species.[5] Luminol has the potential to detect defects in neutrophils ex vivo during oxidative metabolism defects such as chronic granulomatous disease and myeloperoxidase deficiency.[5]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    SHBS0488
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    SHBP9095
    SHBN8149
    SHBN8148

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    Chemiluminescence detection in analytical chemistry
    Lancaster, J. Steven
    Endeavour, 16, 194-194 (1992)
    Yantu Zhang et al.
    Journal of chromatography. A, 1154(1-2), 260-268 (2007-04-24)
    A novel method was developed for the simultaneous determination of glucocorticoid residues such as triamcinolone (TR), prednisolone (PR), hydrocortisone (HC), cortisone (CO), methylprednisolone (MP), dexamethasone (DE) and triamcinolone acetonide (TA) by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection.
    New Developments in Chemiluminescence Research
    Gill, Steven, K.
    Aldrichimica Acta, 16, 59-59 (1983)
    Luminol Chemiluminescence-Delay Method for Determination of Copper(II)
    Kamidate, Tamio; Ishikawa, Atsushi; Watanabe, Hiroto.
    Bulletin of the Chemical Society of Japan, 65, 1591-1591 (1992)
    Opioids prevent regeneration in adult mammals through inhibition of ROS production
    Elodie L, et al.
    Scientific Reports, 8(1) (2018)
    View All Related Papers

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