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About This Item
Empirical Formula (Hill Notation):
C8H7N3O2
CAS Number:
Molecular Weight:
177.16
UNSPSC Code:
12352103
NACRES:
NA.23
PubChem Substance ID:
EC Number:
208-309-4
Beilstein/REAXYS Number:
383929
MDL number:
Product Name
Luminol, 97%
InChI key
HWYHZTIRURJOHG-UHFFFAOYSA-N
InChI
1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)
SMILES string
Nc1cccc2C(=O)NNC(=O)c12
assay
97%
mp
>300 °C (lit.)
Quality Level
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Application
- As a chemiluminiscent substance. Used to measure opsonic and phagocytic function.
- As a biological sensor. Used to detect the polymorphonuclear leukocytes (PMNL) response in a patient with a myeloperoxidase (MPO) deficiency.
- Used as a forensic test for blood.
Luminol has been used in a protocol to study the immune-related responses in Arabidopsis thaliana via luminol/peroxidase-based assay.
Features and Benefits
- Very effective chemiluminiscent compound.
- Can detect sub-part per billion levels of metal ions with simple and inexpensive instrumentation.
Used for chemiluminescence analysis of, for example, metal cations, blood, and glucococorticoids.
General description
Luminol is a very efficient and commonly used chemiluminiscent compound in condensed phase. When basic solutions of luminol containing oxygen are treated with an oxidizing agent, light is produced. Oxidising agents capable of initiating this particular reaction are Nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Luminol-based assay for detection of immunity elicitor-induced hydrogen peroxide production in Arabidopsis thaliana leaves.
Bisceglia NG, et al.
Bio-protocol, 5, e1685-e1685 (2015)
Fast gas chromatography with luminol chemiluminescence detection for the simultaneous determination of nitrogen dioxide and peroxyacetyl nitrate in the atmosphere
Marley, Nancy A., et al.
The Review of Scientific Instruments, 75, 4595-4605 (2004)
T I Quickenden et al.
Luminescence : the journal of biological and chemical luminescence, 16(4), 295-298 (2001-08-21)
A wide range of domestic and industrial substances that might be mistaken for haemoglobin in the forensic luminol test for blood were examined. The substances studied were in the categories of vegetable or fruit pulps and juices; domestic and commercial
Mechanism of cobalt catalysis of luminol chemiluminescence
Burdo, Timothy G., and W. Rudolf Seitz
Analytical Chemistry, 47, 1639-1643 (1975)
C Dahlgren et al.
Infection and immunity, 39(2), 736-741 (1983-02-01)
When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence, linked to activation of the oxidative metabolism of the cells. PMNL isolated from a patient with a myeloperoxidase deficiency were found to produce almost no luminol-dependent
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