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A1603

Sigma-Aldrich

Alginate Lyase

powder, ≥10,000 units/g solid

Synonym(s):

poly-beta-D-mannuronate lyase

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0.2 MG
CN¥7,657.47
1 MG
CN¥15,961.97
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CN¥25,615.51

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0.2 MG
CN¥7,657.47
1 MG
CN¥15,961.97
5 X 1 MG
CN¥25,615.51

About This Item

CAS Number:
9024-15-1
EC Number:
4.2.2.3 (BRENDA, IUBMB)
MDL number:
MFCD03454593
UNSPSC Code:
12352204
NACRES:
NA.54

CN¥7,657.47

Please contact Customer Service for Availability
Request a Bulk Order

form

powder

specific activity

≥10,000 units/g solid

storage temp.

2-8°C

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Monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

FITC conjugate

conjugate

CY3 conjugate

conjugate

biotin conjugate

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

form

buffered aqueous glycerol solution

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

General description

Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.[1]

Application

Alginate Lyase has been used:
  • in the pre-processing of enzyme[2]
  • to de-encapsulate cells[3]
  • to digest Pseudomonas exopolysaccharide to facilitate electroporation[4]

Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture [5].

Biochem/physiol Actions

Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.[1]
Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.

Unit Definition

One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C

Preparation Note

Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000422270
    0000422270
    0000410155
    0000410155
    0000389131

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    Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications
    Zhu B and Yin H
    Bioengineered, 6(3), 125-131 (2015)
    François Thomas et al.
    Environmental microbiology, 14(9), 2379-2394 (2012-04-20)
    Alginate constitutes a significant part of seaweed biomass and thus a crucial nutrient for numerous marine heterotrophic bacteria. However, the mechanisms for alginate assimilation remain largely unknown in marine microorganisms. We show here that the genome of the marine flavobacterium
    Bunzo Mikami et al.
    Acta crystallographica. Section D, Biological crystallography, 68(Pt 9), 1207-1216 (2012-09-06)
    The structures of two mutants (H192A and Y246F) of a mannuronate-specific alginate lyase, A1-III, from Sphingomonas species A1 complexed with a tetrasaccharide substrate [4-deoxy-L-erythro-hex-4-ene-pyranosyluronate-(mannuronate)(2)-mannuronic acid] were determined by X-ray crystallography at around 2.2 Å resolution together with the apo form
    Enzyme-assisted extraction of fucoxanthin and lipids containing polyunsaturated fatty acids from Undaria pinnatifida using dimethyl ether and ethanol
    Billakanti JM, et al.
    Process Biochemistry (Oxford, United Kingdom), 48(12), 1999-2008 (2013)
    Bacteriophages phi MR299-2 and phi NH-4 Can Eliminate Pseudomonas aeruginosa in the Murine Lung and on Cystic Fibrosis Lung Airway Cells
    Alemayehu D, et al.
    mBio, 3(2) (2012)
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