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A9594

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Synonym(s):

Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.32

biological source

mouse

Quality Level

200

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

species reactivity

all

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

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General description

Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.

Learn more product details in our FLAG® application portal.

Physical form

Solution in phosphate buffered saline plus 1% BSA and preservative

Preparation Note

Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

Other Notes

Suggested concentration of 1-10 mg/ml for immunocytochemistry.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Takeshi Yoshizumi et al.
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Genes & development, 33(15-16), 1048-1068 (2019-06-22)
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Oliver Wicht et al.
Journal of virology, 88(9), 4943-4952 (2014-02-21)
Enveloped viruses carry highly specialized glycoproteins that catalyze membrane fusion under strict spatial and temporal control. To prevent premature activation after biosynthesis, viral class I fusion proteins adopt a locked conformation and require proteolytic cleavage to render them fusion-ready. This
Joanna Lawrence et al.
Molecular pharmacology, 67(6), 1822-1828 (2005-03-22)
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Robert S Ohgami et al.
Blood, 108(4), 1388-1394 (2006-04-13)
Iron and copper are essential for all organisms, assuming critical roles as cofactors in many enzymes. In eukaryotes, the transmembrane transport of these elements is a highly regulated process facilitated by the single electron reduction of each metal. Previously, we
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