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A9594

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Synonym(s):

Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk

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0.2 MG
CN¥6,134.36
1 MG
CN¥15,604.28
5 X 1 MG
CN¥21,147.94

CN¥6,134.36

Available to ship onApril 17, 2025Details

New, lower price on this item!
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0.2 MG
CN¥6,134.36
1 MG
CN¥15,604.28
5 X 1 MG
CN¥21,147.94

About This Item

UNSPSC Code:
41106514
NACRES:
NA.32

CN¥6,134.36

Available to ship onApril 17, 2025Details

New, lower price on this item!

biological source

mouse

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

species reactivity

all

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

isotype

IgG1

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F4049F3040F3165
Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

A9594

Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mouse

Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous solution

F4049

Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse

Monoclonal ANTI-FLAG® M1 antibody clone M1, purified immunoglobulin, buffered aqueous solution

F3040

Monoclonal ANTI-FLAG® M1 antibody

Monoclonal ANTI-FLAG® M2 antibody produced in mouse clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

F3165

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone

M2, monoclonal

clone

M2, monoclonal

clone

M1, monoclonal

clone

M2, monoclonal

conjugate

CY3 conjugate

conjugate

FITC conjugate

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin (Purified IgG1 subclass)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

technique(s)

western blot: 10 μg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

General description

Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.

Learn more product details in our FLAG® application portal.

Physical form

Solution in phosphate buffered saline plus 1% BSA and preservative

Preparation Note

Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

Other Notes

Suggested concentration of 1-10 mg/ml for immunocytochemistry.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000364486
    0000343371
    0000364486
    SLCK7207
    SLCD6834

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    Takeshi Yoshizumi et al.
    Biomacromolecules, 19(5), 1582-1591 (2018-03-31)
    Selective gene delivery into organellar genomes (mitochondrial and plastid genomes) has been limited because of a lack of appropriate platform technology, even though these organelles are essential for metabolite and energy production. Techniques for selective organellar modification are needed to
    Oliver Wicht et al.
    Journal of virology, 88(9), 4943-4952 (2014-02-21)
    Enveloped viruses carry highly specialized glycoproteins that catalyze membrane fusion under strict spatial and temporal control. To prevent premature activation after biosynthesis, viral class I fusion proteins adopt a locked conformation and require proteolytic cleavage to render them fusion-ready. This
    Joanna Lawrence et al.
    Molecular pharmacology, 67(6), 1822-1828 (2005-03-22)
    The small GTP-binding protein ADP ribosylation factor 6 (ARF6) has recently been implicated in the internalization of G protein-coupled receptors (GPCRs), although its precise molecular mechanism in this process remains unclear. We have recently identified centaurin alpha(1) as a GTPase
    Yanpu He et al.
    Science advances, 6(24), eaba7589-eaba7589 (2020-06-26)
    The stimulator of interferon (IFN) genes (STING) pathway constitutes a highly important part of immune responses against various cancers and infections. Consequently, administration of STING agonists such as cyclic GMP-AMP (cGAMP) has been identified as a promising approach to target
    Robert S Ohgami et al.
    Blood, 108(4), 1388-1394 (2006-04-13)
    Iron and copper are essential for all organisms, assuming critical roles as cofactors in many enzymes. In eukaryotes, the transmembrane transport of these elements is a highly regulated process facilitated by the single electron reduction of each metal. Previously, we
    View All Related Papers

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