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Merck
CN

P2602

SAFC

Peripheral Blood Medium

liquid, application tested

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CN¥107,440.72

CN¥107,440.72


预计发货时间May 12, 2025详情



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1 EACH
CN¥107,440.72

About This Item

UNSPSC代码:
12352207

CN¥107,440.72


预计发货时间May 12, 2025详情


无菌性

sterile-filtered

表单

liquid

IVD

for in vitro diagnostic use

杂质

endotoxin, tested

适用性

application tested

运输

dry ice

储存温度

−20°C

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OGS3037OGS601OGS245
origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

bacteria selection

kanamycin

bacteria selection

kanamycin

bacteria selection

kanamycin

bacteria selection

kanamycin

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

mol wt

size 5475 bp

mol wt

size 5501 bp

mol wt

size 4533 bp

mol wt

size 4533 bp

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

应用

Peripheral Blood Medium has been developed for in vitro short-term growth of peripheral blood lymphocytes for chromosome analysis.

Peripheral Blood Medium supports mitotic indexes comparable or superior to the best available media. The quality of metaphases and improved chromosome morphology facilitates successful case completion. Because optimized lots of serum and PHA are utilized for production, fluctuation in product quality during manufacturing is minimized. The medium is offered as a complete, ready-to-use frozen solution requiring no supplementation. To ensure optimum results, an insert is provided with each package detailing product specifications and instructions for use.

特点和优势

Development of Sigma′s Peripheral Blood Medium involved extensive R&D work and parallel evaluations in a licensed cytogenetics laboratory. Initial efforts focused on the basal medium formulation with incorporation of components that improved the mitotic index. Subsequent efforts concentrated on the selection of serum and phytohemagglutin (PHA) lots by monitoring mitogenic response in a flow cytometer. Short-term (24 to 72 hours) cultures of human blood specimens were used for all assays. Medium samples were compared to several commercially available and custom-made media routinely used in cytogenetic laboratories. Analyses included chromosome morphology, spreading and band resolution level. Stability studies at various temperatures were used to evaluate medium performance and ensure the best storage temperature.

其他说明

Proprietary formulation containing RPMI-1640 with L-glutamine, HEPES and sodium bicarbonate buffers, fetal bovine serum, phytohemagglutinin (PHA), and gentamicin sulfate.

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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M R O'Donovan et al.
Mutagenesis, 10(4), 371-374 (1995-07-01)
Although lymphocytes in phytohaemagglutinin (PHA)-stimulated whole blood cultures are routinely used to assess genotoxin-induced chromosome damage, very little information is available on the effect of PHA on the various cell populations present, and there appear to be no data for

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