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安全信息

I4506

Sigma-Aldrich

IgG 来源于人类血清

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

别名:

人 IgG

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About This Item

MDL编号:
MFCD00163923
UNSPSC代码:
12352203
NACRES:
NA.46

偶联物

unconjugated

质量水平

300

等级

reagent grade

检测方案

≥95% (SDS-PAGE)

形式

essentially salt-free, lyophilized powder

组成

Protein, ≥90%

技术

ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable

储存温度

2-8°C

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相关类别

一般描述

纯化人IgG由混合的正常人血清经过分级分离和离子交换层析获得。本品以基本不含盐（不到1%钠）的冻干粉形式提供。

应用

以人血清IgG为标准品，在ELISA法测定中采用浓度为1.274 mg/ml, 检测牙 龈卟啉单胞菌 和 间质普雷沃氏菌的抗体反应。它被用在0.2 mg/ml浓度时对Vero、BHK、PtK2细胞的免疫标记。人血清IgG被用作抗-志贺氏菌 LPS抗体的SDS-PAGE的标准品。

生化/生理作用

IgG抗体亚型是免疫系统中最丰富的血清免疫球蛋白。它是由B细胞分泌的，存在于血液和细胞外液体中，可以防止细菌、真菌和病毒引起的感染。母体IgG通过胎盘传递给胎儿，该过程对新生儿免疫防御感染至关重要。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物应用。

相关产品

产品编号
说明
价格

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Is Product I4506, IgG from human serum, tested for endotoxin?

    No. Product No. I4506 is not tested for endotoxin levels. 

  4. What is the extinction coefficient forProduct I4506, human IgG?

    Protein concentration can be determined after reconstitution by absorbance at 280 nm (E280 (1%) = 14).  A 1% solution is 10 mg/mL.

  5. How can I use Product I4506, IgG from human serum, to block Fc receptors on the cell surface for flow cytometry analysis of cells?

    Human cells to be used for FACS staining may be Fc-blocked by treatment with 1 μg of human IgG per 100,000 cells, for 15 minutes at room temperature. It is not necessary to wash to remove excess IgG before proceeding with staining.

  6. Is Product I4506, IgG from human serum, from a single donor?

    No. Product No. I4506 is from pooled normal serum.

  7. How should I store my solution of Product I4506, IgG from human serum?

    Solutions can be stored at -20 °C for at least 1 year.

  8. What isotype is present in Product I4506, IgG from human serum?

    Product No. I4506 is IgG, not a myeloma protein.  It contains all the IgG isotypes.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nils A Brechmann et al.
Biotechnology progress, 35(3), e2775-e2775 (2019-01-11)
High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum
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Journal of cell science, 130(24), 4168-4179 (2017-11-09)
Phagosome formation is a complicated process that requires spatiotemporally regulated actin reorganization. We found that RhoC GTPase is a critical regulator of FcγR-mediated phagocytosis in macrophages. Our live-cell imaging revealed that RhoC, but not RhoA, is recruited to phagocytic cups
Katinka Döhner et al.
Molecular biology of the cell, 13(8), 2795-2809 (2002-08-16)
After fusion of the viral envelope with the plasma membrane, herpes simplex virus type 1 (HSV1) capsids are transported along microtubules (MTs) from the cell periphery to the nucleus. The motor ATPase cytoplasmic dynein and its multisubunit cofactor dynactin mediate
Samuel Pontes-Quero et al.
Nature communications, 10(1), 2016-2016 (2019-05-03)
Appropriate therapeutic modulation of endothelial proliferation and sprouting is essential for the effective inhibition of angiogenesis in cancer or its induction in cardiovascular disease. The current view is that an increase in growth factor concentration, and the resulting mitogenic activity
Elisa Lázaro-Ibáñez et al.
Journal of extracellular vesicles, 8(1), 1656993-1656993 (2019-09-10)
Extracellular vesicles have the capacity to transfer lipids, proteins, and nucleic acids between cells, thereby influencing the recipient cell's phenotype. While the role of RNAs in EVs has been extensively studied, the function of DNA remains elusive. Here, we distinguished
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