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I4506

IgG from human serum

Name: IgG from human serum
Brand: SIGMA

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym(s):

Human IgG

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00163923

Conjugate:

unconjugated

Technique(s):

ELISA: suitable, dot immunobinding: suitable, immunoelectrophoresis: suitable, western blot: suitable

Application:

DIBA, ELISA, IEP, WB

Citations:

228

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Properties

conjugate

unconjugated

Quality Level

300

grade

reagent grade

assay

≥95% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

composition

Protein, ≥90%

technique(s)

ELISA: suitable, dot immunobinding: suitable, immunoelectrophoresis: suitable, western blot: suitable

storage temp.

2-8°C

Description

General description

Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.

Application

IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia. It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml. IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

高风险级别生物产品--人源产品

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Certificates of Analysis (COA)

Lot/Batch Number

0000502382

0000498489

0000376188

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Protocols

Released N-Linked Glycan Analysis Workflow of Adalimumab

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

Articles

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

HILIC Stationary Phase for Glycan Profiling

Glycan profiling of monoclonal antibodies using Ascentis^® Express ZIC^®-cHILIC columns enables high-resolution IgG glycan separation with improved efficiency and speed.

Global Trade Item Number

SKU	GTIN
I4506-500MG	04061833856864
I4506-100MG	04061833856840
I4506-10MG	04061833856857
I4506-50MG	04061833856871