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I4506

Sigma-Aldrich

IgG from human serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym(s):

Human IgG

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About This Item

MDL number:
MFCD00163923
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

unconjugated

Quality Level

300

grade

reagent grade

Assay

≥95% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

composition

Protein, ≥90%

technique(s)

ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable

storage temp.

2-8°C

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General description

Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.

Application

IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia. It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml. IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

related product

Product No.
Description
Pricing

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Is Product I4506, IgG from human serum, tested for endotoxin?

    No. Product No. I4506 is not tested for endotoxin levels. 

  4. What is the extinction coefficient forProduct I4506, human IgG?

    Protein concentration can be determined after reconstitution by absorbance at 280 nm (E280 (1%) = 14).  A 1% solution is 10 mg/mL.

  5. How can I use Product I4506, IgG from human serum, to block Fc receptors on the cell surface for flow cytometry analysis of cells?

    Human cells to be used for FACS staining may be Fc-blocked by treatment with 1 μg of human IgG per 100,000 cells, for 15 minutes at room temperature. It is not necessary to wash to remove excess IgG before proceeding with staining.

  6. Is Product I4506, IgG from human serum, from a single donor?

    No. Product No. I4506 is from pooled normal serum.

  7. How should I store my solution of Product I4506, IgG from human serum?

    Solutions can be stored at -20 °C for at least 1 year.

  8. What isotype is present in Product I4506, IgG from human serum?

    Product No. I4506 is IgG, not a myeloma protein.  It contains all the IgG isotypes.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Katinka Döhner et al.
Molecular biology of the cell, 13(8), 2795-2809 (2002-08-16)
After fusion of the viral envelope with the plasma membrane, herpes simplex virus type 1 (HSV1) capsids are transported along microtubules (MTs) from the cell periphery to the nucleus. The motor ATPase cytoplasmic dynein and its multisubunit cofactor dynactin mediate
Nils A Brechmann et al.
Biotechnology progress, 35(3), e2775-e2775 (2019-01-11)
High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum
Youhei Egami et al.
Journal of cell science, 130(24), 4168-4179 (2017-11-09)
Phagosome formation is a complicated process that requires spatiotemporally regulated actin reorganization. We found that RhoC GTPase is a critical regulator of FcγR-mediated phagocytosis in macrophages. Our live-cell imaging revealed that RhoC, but not RhoA, is recruited to phagocytic cups
Fares Nigim et al.
Targeted oncology, 14(4), 479-489 (2019-07-14)
High-grade meningiomas (HGMs; World Health Organization [WHO] classification grade II and III) have high relapse rates and poor clinical outcomes despite surgery and radiation treatments. No effective medical therapy currently exists for HGMs, and developing novel therapeutic strategies depends on
Egil S Blix et al.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 21(5), 840-847 (2015-02-18)
Multiple myeloma (MM) is considered an incurable B cell malignancy, although many patients can benefit from high-dose therapy with autologous stem cell transplantation (ASCT) as a first-line treatment. In non-Hodgkin lymphoma (NHL), ASCT is usually performed after relapse with curative
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