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Merck
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A2767

Sigma-Aldrich

腺苷5′-三磷酸-琼脂糖

lyophilized powder

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别名:
5′-ATP agarose
MDL编号:
MFCD00801332
UNSPSC代码:
41106500
eCl@ss:
32160414
NACRES:
NA.56

生物来源

plant (Sea weed)

质量水平

200

形式

lyophilized powder

标记范围

1-5 μmol per mL

基质

cross-linked 4% beaded agarose

基质活化

cyanogen bromide

基质附着

C-8

基质隔离区

9 atoms

储存温度

−20°C

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相关类别

一般描述

5′-三磷酸腺苷-琼脂糖（5′-ATP-琼脂糖）是5-ATP通过5′-ATP的C-8原子与交联4%珠状琼脂糖形成的缀合物（由溴化氰激活）。5′-ATP–琼脂糖适用于各种蛋白质和酶的亲和纯化，如周期蛋白依赖性激酶2（CDK2）、热休克蛋白和隐花色素。

应用

5′-三磷酸腺苷-琼脂糖（5′-ATP–琼脂糖）适用于各种蛋白质和酶的亲和纯化，如周期蛋白依赖性激酶2（CDK2）、热休克蛋白-70（HSP-70）和隐花色素。5′-ATP琼脂糖已用于亲和色谱中，以从艾氏腹水癌肿瘤细胞中纯化尿苷激酶。
腺苷5′-三磷酸琼脂糖（5′-ATP琼脂糖）已用于亲和色谱中，以从艾氏腹水癌肿瘤细胞中纯化尿苷激酶。

外形

用乳糖稳定化的冻干粉

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How can I hydrate Product A2767, Adenosine 5´-triphosphate-Agarose?

    The resin can be hydrated by placing it in excess water, approximately 50 mL per g of resin, for at least 30 minutes.  Remove the lactose stabilizer by washing the resin on a Buchner funnel with gentle vacuum, using approximately 100 mL of water per g of resin.  Do not allow the resin to dry.  Resuspend the resin in excess water or starting buffer to pack the column bed.

  6. How can I elute my proteins from Product A2767, Adenosine 5´-triphosphate-Agarose?

    Specifically bound proteins can be eluted with 10-100 mM ATP or ADP. Nonspecifically bound proteins can be eluted with 2 M NaCl or KCl in water or 7 M urea.

  7. How can I regenerate Product A2767, Adenosine 5´-triphosphate-Agarose?

    Since N6-[N-(6-aminohexyl)-carbamyl]-ADP is a substrate for acetate kinase and pyruvate kinase, these enzymes can be used to regenerate ATP from ADP on the resin under conditions favoring the reverse reaction. Wash the resin with 20 volumes of 50 mM Tris HCl buffer (pH 8.2), with 0.2 mM EDTA and 100 mM KCl.  Incubate the resin overnight at 2-8°C in an ATP-regenerating mixture containing 0.2 mM phosphoenol pyruvate, pyruvate kinase (10 units per mL of resin), 5 mM MgCl2, 0.2 mM EDTA and 100 mM KCl in 50 mM Tris HCl buffer (pH 8.2). Wash the resin with 25 column volumes of 2 mM ATP in 2 M KCl and reequilibrate the resin with 25 column volumes of sample buffer. Alternatively, the ATP regenerating mixture can contain 20 mM acetyl phosphate, acetate kinase (6.8 units per mL of resin), and 3 mM MgCl2 in 100 mM Tris HCl (pH 7.6). If acetate kinase is used, prewash the resin with 25 column volumes of 100 mM Tris HCl (pH 7.6), but use the same final wash and reequilibration steps as given for pyruvate kinase.

  8. Can I store Product A2767, Adenosine 5´-triphosphate-Agarose, after I have hydrated it?

    For the short term, it is possible to store the hydrated resin in 20% ethanol and dilute buffer at 2-8°C.  In general, the hydrated resin can be stored refrigerated in water or buffer containing a bacteriostat, such as 0.02% sodium azide or thimerosal. Do not autoclave or freeze the hydrated resin. The resin can be used several times without loss of effectiveness. However, the ATP will slowly hydrolyze to ADP over time, and under certain conditions this process may be accelerated during usage.  The resin can be regenerated; please see the regeneration FAQ for this product.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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