Skip to Content
Merck
CN
All Photos(3)

Key Documents

View All Documentation

A2767

Sigma-Aldrich

Adenosine 5′-triphosphate–Agarose

lyophilized powder

Synonym(s):

5′-ATP agarose

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

MDL number:
MFCD00801332
UNSPSC Code:
41106500
eCl@ss:
32160414
NACRES:
NA.56

biological source

plant (Sea weed)

Quality Level

200

form

lyophilized powder

extent of labeling

1-5 μmol per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

C-8

matrix spacer

9 atoms

storage temp.

−20°C

SMILES string

[X]Nc1ncnc2[n](cnc21)[C@@H]3O[C@@H]([C@H]([C@H]3O)O)CO[P](=O)(O[P](=O)(O[P](=O)(O)O)O)O

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Adenosine 5′-triphosphate–Agarose (5′-ATP-agarose) is a conjugate of 5′-ATP to crosslinked 4% beaded agarose (activated by cyanogen bromide), via the C-8 atom of 5′-ATP. 5′-ATP–agarose is applicable for affinity purification of various proteins and enzymes like cyclin-dependent kinase 2 (CDK2), heat shock proteins, and cryptochromes.

Application

Adenosine 5′-triphosphate Agarose (5′-ATP agarose) has been used in affinity chromatography to purify uridine kinase from Ehrlich ascites tumor cells.
Adenosine 5′-triphosphate–Agarose (5′-ATP–agarose) is applicable for affinity purification of various proteins and enzymes like cyclin-dependent kinase 2 (CDK2), heat shock proteins-70 (HSP-70), and cryptochromes. 5′-ATP agarose has been used in affinity chromatography to purify uridine kinase from Ehrlich ascites tumor cells.

Physical form

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000320291
0000320291
0000271381
0000271381
SLBT6809

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How can I hydrate Product A2767, Adenosine 5´-triphosphate-Agarose?

    The resin can be hydrated by placing it in excess water, approximately 50 mL per g of resin, for at least 30 minutes.  Remove the lactose stabilizer by washing the resin on a Buchner funnel with gentle vacuum, using approximately 100 mL of water per g of resin.  Do not allow the resin to dry.  Resuspend the resin in excess water or starting buffer to pack the column bed.

  6. How can I elute my proteins from Product A2767, Adenosine 5´-triphosphate-Agarose?

    Specifically bound proteins can be eluted with 10-100 mM ATP or ADP. Nonspecifically bound proteins can be eluted with 2 M NaCl or KCl in water or 7 M urea.

  7. How can I regenerate Product A2767, Adenosine 5´-triphosphate-Agarose?

    Since N6-[N-(6-aminohexyl)-carbamyl]-ADP is a substrate for acetate kinase and pyruvate kinase, these enzymes can be used to regenerate ATP from ADP on the resin under conditions favoring the reverse reaction. Wash the resin with 20 volumes of 50 mM Tris HCl buffer (pH 8.2), with 0.2 mM EDTA and 100 mM KCl.  Incubate the resin overnight at 2-8°C in an ATP-regenerating mixture containing 0.2 mM phosphoenol pyruvate, pyruvate kinase (10 units per mL of resin), 5 mM MgCl2, 0.2 mM EDTA and 100 mM KCl in 50 mM Tris HCl buffer (pH 8.2). Wash the resin with 25 column volumes of 2 mM ATP in 2 M KCl and reequilibrate the resin with 25 column volumes of sample buffer. Alternatively, the ATP regenerating mixture can contain 20 mM acetyl phosphate, acetate kinase (6.8 units per mL of resin), and 3 mM MgCl2 in 100 mM Tris HCl (pH 7.6). If acetate kinase is used, prewash the resin with 25 column volumes of 100 mM Tris HCl (pH 7.6), but use the same final wash and reequilibration steps as given for pyruvate kinase.

  8. Can I store Product A2767, Adenosine 5´-triphosphate-Agarose, after I have hydrated it?

    For the short term, it is possible to store the hydrated resin in 20% ethanol and dilute buffer at 2-8°C.  In general, the hydrated resin can be stored refrigerated in water or buffer containing a bacteriostat, such as 0.02% sodium azide or thimerosal. Do not autoclave or freeze the hydrated resin. The resin can be used several times without loss of effectiveness. However, the ATP will slowly hydrolyze to ADP over time, and under certain conditions this process may be accelerated during usage.  The resin can be regenerated; please see the regeneration FAQ for this product.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

M Brungs et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(1), 107-111 (1995-01-03)
5-Lipoxygenase (5-LO; EC 1.13.11.34) activity in the human monocytic cell line Mono Mac 6 was upregulated by combined treatment with transforming growth factor beta 1 (TGF-beta) and 1,25-dihydroxyvitamin D3 (VD3). In undifferentiated cells, 5-LO enzyme activity was undetectable. After the
F Geller et al.
Journal of ethnopharmacology, 128(3), 561-566 (2010-02-13)
Preparation from leaves of Cordia americana have been widely used in traditional medicine in South Brazil to treat wounds and various inflammations. The objective of this work was to identify the effective compounds in the ethanolic extract prepared from the
L Fischer et al.
British journal of pharmacology, 152(4), 471-480 (2007-08-21)
Licofelone is a dual inhibitor of the cyclooxygenase and 5-lipoxygenase (5-LO) pathway, and has been developed for the treatment of inflammatory diseases. Here, we investigated the molecular mechanisms underlying the inhibition by licofelone of the formation of 5-LO products. The
C Prodromou et al.
The EMBO journal, 18(3), 754-762 (1999-02-02)
The in vivo function of the heat shock protein 90 (Hsp90) molecular chaperone is dependent on the binding and hydrolysis of ATP, and on interactions with a variety of co-chaperones containing tetratricopeptide repeat (TPR) domains. We have now analysed the
C Greiner et al.
British journal of pharmacology, 164(2b), 781-793 (2011-04-22)
5-Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of pro-inflammatory leukotrienes (LTs) representing a potential target for pharmacological intervention with inflammation and allergic disorders. Although many LT synthesis inhibitors are effective in simple in vitro test systems, they frequently
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service