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HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Discovery® BIO Wide Pore C18 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

Cytochrome c from pigeon breast muscle

≥95% based on Mol. Wt. 12,173 basis

Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis

Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

Cytochrome c from equine heart

suitable for GFC marker, BioReagent

Cytochrome c from equine heart

≥95% based on Mol. Wt. 12,384 basis

Cytochrome c from equine heart

≥95% (SDS-PAGE)

Cytochrome c from Saccharomyces cerevisiae

≥85% based on Mol. Wt. 12,588 basis

Cytochrome c from human heart

≥95% (SDS-PAGE), lyophilized powder

Cytochrome c from bovine heart

≥95% (GE)

Cytochrome c from equine heart

BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

CONDITIONS

column

Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)

mobile phase

[A] (0.1% TFA in water):(0.1% TFA in acetonitrile); (70:30), [B] (0.1% TFA in water):(0.1% TFA in acetonitrile), 64:36

gradient

0-100% B in 30 min

flow rate

1 mL/min

column temp.

ambient

detector

UV, 220 nm

injection

12 μL each at 0.8 mg/mL in 0.1%TFA

Description

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany