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Supelco

Discovery® BIO Wide Pore C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm

Synonym(s):

Fully Porous Wide Pore C18 HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® BIO Wide Pore C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

9.2% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

100 m2/g

surface coverage

3.6 μmol/m2

matrix

silica particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

300 Å

operating pH

2-8

separation technique

reversed phase

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General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Application


  • Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals.: This study provides a detailed kinetic performance comparison of different types of reversed-phase HPLC columns, including the Discovery® BIO Wide Pore C18 HPLC Column. The research highlights its efficiency and resolution in separating complex protein biopharmaceuticals, making it a valuable tool for analytical chemists in biopharmaceutical analysis (Jaag et al., 2022).

  • RP-LC x RP-LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases.: This paper investigates the use of reverse-phase liquid chromatography (RP-LC) with both totally porous and partially porous stationary phases, including the BIO Wide Pore C18 HPLC Column, to analyze tryptic digests. The findings demonstrate its robustness and high resolution in proteomics applications, enabling precise peptide mapping and protein characterization (Mondello et al., 2010).

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Product No.
Description
Pricing

G001593

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #2), application for HPLC

G001585

HPLC Analysis of Angiotensins on Discovery® BIO Wide Pore C18, application for HPLC

G001719

HPLC Analysis of Carboxymethylated Apohemoglobin (Horse) Tryptic Digest on Discovery® BIO Wide Pore C18, application for HPLC

G001505A

HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C18, application for HPLC

G001581

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18, application for HPLC

G001512

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1), application for HPLC

G001727

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 28-38 Min. Window, application for HPLC

G001730

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window, application for HPLC

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guard cartridge

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Certificates of Analysis (COA)

Lot/Batch Number
290567-05
290567-04
290567-03
290567-02
290567-01

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Articles

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Supelco Preparative HPLC products for Pharmaceutical Development and Production

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