Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

ABSTRACT

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

MATERIALS

Product Number

Brand

Product Description

517747

Sigma-Aldrich

Aluminum oxide, mesoporous, MSU-X (wormhole), average pore size 3.8 nm

T5525

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3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet

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D-(+)-Trehalose dihydrate, ≥98.5% (HPLC), from Saccharomyces cerevisiae

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3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)

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