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T0167

Sigma-Aldrich

D-(+)-Trehalose dihydrate

from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

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Synonym(s):
α,α-Trehalose, α-D-Glucopyranosyl-α-D-glucopyranoside
Empirical Formula (Hill Notation):
C12H22O11 · 2H2O
CAS Number:
6138-23-4
Molecular Weight:
378.33
Beilstein:
5322018
EC Number:
202-739-6
MDL number:
MFCD00071594
UNSPSC Code:
12352201
PubChem Substance ID:
24899871
NACRES:
NA.75

Quality Level

200

biological source

Saccharomyces cerevisiae

product line

BioReagent

Assay

≥99%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

solubility

H2O: 50 mg/mL

SMILES string

[H]O[H].[H]O[H].OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12;;/h3-20H,1-2H2;2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-;;/m1../s1

InChI key

DPVHGFAJLZWDOC-PVXXTIHASA-N

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General description

Trehalose is a non-reducing disaccharide, ubiquitously present in nature. It is used as a food preservative and also to retain enzymes, vaccines, cells and many other molecules. Trehalose is present as hemolymph sugar among the invertebrates.

Application

D-(+)-Trehalose dihydrate has been used in a slow-freezing method for freezing rabbit oocytes. It has also been used in solid-surface vitrification (SSV) of in vitro-matured oocytes. It is suitable for use as a cryoprotectant in a variety of cell freezing media.

Biochem/physiol Actions

Trehalose is essential for embryogenesis, carbon metabolism, photosynthesis and flowering in plants. It predominantly exists among the species that supports anhydrobiosis (life without water). It serves as a carbohydrate reserve in microorganisms and protects them from adverse conditions, such as heat, cold, dehydration, desiccation and oxidation. Trehalose acts as a chemical chaperone affecting protein folding. It is known to prevent amyloid generation and accummulation of β-amyloid in vitro. A similar effect of trehalose has been observed with huntingtin protein aggregation. Thus, it might serve as a protective agent in Alzheimer′s disease and Huntington′s disease.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

涉药品监管产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can solutions of Product T0167, D-(+)-Trehalose dihydrate, be autoclaved?

    Do not autoclave. It is best to sterile filter solutions.

  6. Do we have information of any fluorescent impurities in Product T0167, D-(+)-Trehalose dihydrate?

    This product is not tested for the presence or absence of fluorescent impurities.

  7. Can Product T0167, D-(+)-Trehalose dihydrate, be used as a substitute for protein in a freezing media?

    Yes, it should work as a substitute for protein in a freezing media.  It is commonly used at a final concentration of 5% (50 mg/mL).  Alternatively, one could use methylcellulose.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Trehalose metabolism: from osmoprotection to signaling
Iturriaga G, et al.
International Journal of Molecular Sciences, 10(9), 3793-3810 (2009)
Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions.
El-Bashiti T, et al.
Plant Science, 169(1), 47-54 (2005)
Production of good-quality porcine blastocysts by in vitro fertilization of follicular oocytes vitrified at the germinal vesicle stage
Somfai T, et al.
Theriogenology, 73, 147-156 (2010)
Developmental competence of in vitro-fertilized porcine oocytes after in vitro maturation and solid surface vitrification: effect of cryopreservation on oocyte antioxidative system and cell cycle stage
Somfai T, et al.
Cryobiology, 55(2), 115-126 (2007)
Shipra Malik et al.
MethodsX, 7, 101115-101115 (2020-11-05)
Peptide nucleic acids (PNAs) have emerged as one of the most versatile tools with a wide range of biomedical applications including antisense, antimiR, antigene, as well as site-specific gene editing. The application and potential of PNAs has been limited due
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