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L4777

Sigma-Aldrich

Lipase acrylic resin

greener alternative

≥5,000 U/g, recombinant, expressed in Aspergillus niger

Synonym(s):

Novozyme 435

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About This Item

CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00217536
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in Aspergillus niger

Quality Level

200

form

solid

specific activity

≥5,000 U/g

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

matrix

macroporous acrylic resin

application(s)

diagnostic assay manufacturing

greener alternative category

Enabling,

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

General description

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Lipase from produced by submerged fermentation of a genetically modified Aspergillus niger microorganism and adsorbed on a macroporous resin.

Application

Lipase acrylic resin has been used:
  • as a catalyst to carry out the esterification of pLACT with butyric acid
  • as an additive in fully hydrogenated canola oil (FHCO) and high oleic sunflower oil (HOSO) blend for enzymatic interesterification
  • to study its effect on the degree of oleic acid conversion upon esterification

Immobilized preparation of a thermostable lipase, particularly useful in the synthesis of esters and amides, and has a broad substrate specificity.

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis. Candida antarctica lipase B is considered as an important enzyme due to its use in esterification, hydrolysis, aminolysis and transesterification.

Other Notes

The material may be reused for identical reactions. It is not recommended for use in different reactions due to the probability of cross-contamination and loss of activity. There is no estimation as to the number of times the product may be used.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
0000349293
0000349294
0000342258
0000316693
0000316694

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What solvents are most compatible with Product L4777, Lipase acrylic resin?

    This resin works well in solvent-free reactions, as well as in inert water immiscible solvents such as petroleum ether, n-hexane and n-heptane. If the reaction being catalyzed is hydrolysis of an ester, addition of water to the reaction mix is recommended. For other reactions water is likely not required, and in some cases would actually be detrimental.

  5. What is the substrate specificity of Product L4777, Lipase acrylic resin?

    Lipase acrylic resin has a broad substrate specificity. It promotes reactions between a wide range of primary and secondary alcohols and carboxylic acids.

  6. What are the components of Product L4777, Lipase acrylic resin, and what are the optimal recommended conditions?

    This product consists of a thermostable lipase enzyme immobilized on a macroporous acrylic bead resin. The bead-shaped particles have a diameter in the range of 0.3-0.9 mm. The resin will be compatible with stearyl alcohol at temperatures of 40-60°C, which is the normal operating temperature of the enzyme. This immobilized enzyme can be used in hexane with no adverse effects.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Covalent immobilization of Candida antarctica lipase B on nanopolystyrene and its application to microwave-assisted esterification
Bukhari A, et al.
Chinese Journal of Catalysis, 35(9), 1555-1564 (2014)
Marine fungal and bacterial isolates for lipase production: A comparative study
Advances in Food and Nutrition Research, 78, 71-94 (2016)
Hua Zhao et al.
RSC advances, 8(63), 36025-36033 (2018-10-23)
Enzymatic ring-opening polymerization (ROP) is a benign method for preparing polyesters, such as polylactides and other polylactones. These reactions are typically carried out at relatively high temperatures (60-130 °C), however, there is a deficiency of enzyme-compatible solvents for such thermally-demanding
Bartłomiej Zieniuk et al.
Biotechnology letters, 43(3), 601-612 (2020-10-27)
The aim of the study was to evaluate the possibility of using Y. lipolytica biomass as a whole-cell catalyst in the synthesis of lipophilic antioxidants, with the example of esterification of five phenolic acids with 1-butanol. Freeze-dried Y. lipolytica biomass
Euna Choi et al.
BMC research notes, 15(1), 370-370 (2022-12-13)
Staphylococcus epidermidis is a skin colonizer and a major cause of nosocomial infections that can lead to sepsis. It causes opportunistic infections by forming biofilms on medical devices, which are hard to control with conventional antibiotics. In an attempt to
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