Skip to Content
Merck
CN
  • Metabolite profile, antioxidant capacity, and inhibition of digestive enzymes in infusions of peppermint (Mentha piperita) grown under drought stress.

Metabolite profile, antioxidant capacity, and inhibition of digestive enzymes in infusions of peppermint (Mentha piperita) grown under drought stress.

Journal of agricultural and food chemistry (2014-12-03)
Marely G Figueroa-Pérez, Nuria Elizabeth Rocha-Guzmán, Iza F Pérez-Ramírez, Edmundo Mercado-Silva, Rosalía Reynoso-Camacho
ABSTRACT

Peppermint (Mentha piperita) infusions represent an important source of antioxidants, which can be enhanced by inducing abiotic stress in plants. The aim of this study was to evaluate the effect of drought stress on peppermint cultivation as well as the metabolite profile, antioxidant capacity, and inhibition of digestive enzymes of resulting infusions. At 45 days after planting, irrigation was suppressed until 85 (control), 65, 35, 24, and 12% soil moisture (SM) was reached. The results showed that 35, 24, and 12% SM decreased fresh (20%) and dry (5%) weight. The 35 and 24% SM treatments significantly increased total phenolic and flavonoid contents as well as antioxidant capacity. Coumaric acid, quercetin, luteolin, and naringenin were detected only in some drought treatments; however, in these infusions, fewer amino acids and unsaturated fatty acids were identified. The 24 and 12% SM treatments slightly improved inhibition of pancreatic lipase and α-amylase activity. Therefore, induction of moderate water stress in peppermint is recommended to enhance its biological properties.

MATERIALS
Product Number
Brand
Product Description

USP
Vanillin Melting Point Standard, United States Pharmacopeia (USP) Reference Standard
Supelco
Mettler-Toledo Calibration substance ME 51143093, Vanillin, traceable to primary standards (LGC)
Sigma-Aldrich
Vanillin, ≥97%, FCC, FG
Sigma-Aldrich
Vanillin, ReagentPlus®, 99%
Sigma-Aldrich
Quercetin, ≥95% (HPLC), solid
Sigma-Aldrich
(±)-Naringenin, ≥95%
Sigma-Aldrich
Vanillin, natural, ≥97%, FCC, FG
Sigma-Aldrich
Eriocitrin, ≥98.0% (HPLC)
Supelco
Vanillin, Pharmaceutical Secondary Standard; Certified Reference Material
Eriocitrin, primary reference standard
Vanillin, European Pharmacopoeia (EP) Reference Standard
Supelco
Vanillin melting point standard, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Naringenin, natural (US), 98%
Supelco
(±)-Naringenin, analytical standard
Sigma-Aldrich
Vanillin, tested according to Ph. Eur.
USP
Quercetin, United States Pharmacopeia (USP) Reference Standard
Supelco
Quercetin, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Vanillin, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
Luteolin, analytical standard
Sigma-Aldrich
Hesperidin, ≥80% (HPLC)
Luteolin, primary reference standard
Hesperidin, primary reference standard
Sigma-Aldrich
Luteolin, ≥98% (TLC), powder
Sigma-Aldrich
4-Nitrophenyl butyrate, ≥98%
Supelco
Sodium Nitroprusside, Pharmaceutical Secondary Standard; Certified Reference Material
Hesperidin, European Pharmacopoeia (EP) Reference Standard
Supelco
Hesperidin, analytical standard