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  • An Interactive Database for the Assessment of Histone Antibody Specificity.

An Interactive Database for the Assessment of Histone Antibody Specificity.

Molecular cell (2015-07-28)
Scott B Rothbart, Bradley M Dickson, Jesse R Raab, Adrian T Grzybowski, Krzysztof Krajewski, Angela H Guo, Erin K Shanle, Steven Z Josefowicz, Stephen M Fuchs, C David Allis, Terry R Magnuson, Alexander J Ruthenburg, Brian D Strahl
ABSTRACT

Access to high-quality antibodies is a necessity for the study of histones and their posttranslational modifications (PTMs). Here we debut the Histone Antibody Specificity Database (http://www.histoneantibodies.com), an online and expanding resource cataloging the behavior of widely used, commercially available histone antibodies by peptide microarray. This interactive web portal provides a critical resource to the biological research community that routinely uses these antibodies as detection reagents for a wide range of applications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10, ascites fluid, clone 3HH4-4C10, from mouse
Sigma-Aldrich
Anti-trimethyl (Lys9)-phospho (Ser10)-Histone H3 Antibody, rabbit monoclonal, Upstate®, from rabbit
Sigma-Aldrich
Anti-acetyl-Histone H4 (Lys12) Antibody, serum, Upstate®
Sigma-Aldrich
Anti-acetyl-Histone H4 Antibody, serum, Upstate®
Sigma-Aldrich
Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002, clone RR002, Upstate®, from mouse
Sigma-Aldrich
Anti-trimethyl-Histone H3 (Lys4) Antibody, Upstate®, from rabbit
Sigma-Aldrich
Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, rabbit monoclonal, culture supernatant, clone NL59, Upstate®
Sigma-Aldrich
Anti-trimethyl-Histone H3 (Lys9) Antibody, Upstate®, from rabbit
Sigma-Aldrich
Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonal, culture supernatant, from rabbit
Sigma-Aldrich
Anti-monomethyl-Histone H3 (Lys4) Antibody, Upstate®, from rabbit
Sigma-Aldrich
Anti-trimethyl-Histone H3 (Lys27) Antibody, Upstate®, from rabbit
Sigma-Aldrich
Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal, culture supernatant, clone MC463, Upstate®
Sigma-Aldrich
Anti-acetyl-Histone H4 (Lys5) Antibody, serum, from rabbit