05-1355
Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10
ascites fluid, clone 3HH4-4C10, from mouse
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Synonym(s):
H4KAc, Histone H4 (acetyl), H4 histone family, member N, H4 histone, family 2, histone 2, H4, histone 2, H4a, histone IV, family 2, histone cluster 2, H4a
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
3HH4-4C10, monoclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
acetylation (Lys5/Lys8/Lys12/Lys16)
Gene Information
human ... H4C1(8359)
mouse ... H4C1(326619)
General description
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Posttranslational acetylation of the conserved core histone N-terminal tail domains is typically associated with gene activation.
Specificity
This antibody recognizes Histone H4.
Immunogen
Epitope: Lys5/8/12/16
Linear peptide corresponding to human Histone H4 acetylated at Lys5/8/12/16.
Application
Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10 (mouse monoclonal antibody) validated in WB, ICC to detect acetyl Histone H4 (Lys5/8/12/16) also known as H4KAc, Histone H4 (acetyl).
Quality
Evaluated by Western Blot in HeLa acid extract lysate.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.
Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.
Target description
~ 13 kDa
Physical form
Unpurified
Unpurified mouse monoclonal IgG1κ preservative-free ascites.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa acid extract lysate
HeLa acid extract lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jingwen Feng et al.
Scientific reports, 7(1), 9581-9581 (2017-08-31)
High-content screening is commonly used in studies of the DNA damage response. The double-strand break (DSB) is one of the most harmful types of DNA damage lesions. The conventional method used to quantify DSBs is γH2AX foci counting, which requires
Madhumanti Barman et al.
Frontiers in microbiology, 12, 759817-759817 (2021-12-07)
The apicomplexan parasite, Theileria annulata, is the most prevalent hemoprotozoan in livestock, causing significant economic losses worldwide. It is essential to develop new and improved therapeutics, as current control measures are compromised by the development of resistance against the only
Yolanda Colino-Sanguino et al.
iScience, 21, 773-788 (2019-11-16)
Acetylation of the histone variant H2A.Z (H2A.Zac) occurs at active regulatory regions associated with gene expression. Although the Tip60 complex is proposed to acetylate H2A.Z, functional studies suggest additional enzymes are involved. Here, we show that p300 acetylates H2A.Z at
William Newhard et al.
SLAS discovery : advancing life sciences R & D, 26(5), 642-654 (2021-01-12)
Antiretroviral therapy is able to effectively control but not eradicate HIV infection, which can persist, leading to the need for lifelong therapy. The existence of latently HIV-infected cells is a major barrier to the eradication of chronic HIV infection. Histone
Maxime Wery et al.
RNA (New York, N.Y.), 24(2), 196-208 (2017-11-09)
Antisense transcription can regulate sense gene expression. However, previous annotations of antisense transcription units have been based on detection of mature antisense long noncoding (aslnc)RNAs by RNA-seq and/or microarrays, only giving a partial view of the antisense transcription landscape and
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