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G1007494

UHPLC Analysis of Nucleosides on Purospher® STAR RP-18 endcapped

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Hibar Purospher® STAR RP-18 endcapped, 150 x 4.6 mm, 3 μm (1.50470.0001)
mobile phase: [A] 30 mM ammonium phosphate, pH 5.3 with ammonium hydroxide in water; [B] Methanol; 97.5 [A] / 2.5 [B]
flow rate: 1.0 mL/min
pressure: 3060 psi 211 Bar
column temp.: 25 °C
detector: UV 254
injection: 5 μL
sample: 5 μg/mL of 12 nucleosides in 98:2 30mM ammonium phosphate pH 5.3 with ammonium hydroxide in water: methanol

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

Nucleosides are structural subunits of nucleic acids, the macromolecules that convey genetic information in living cells. They contain a molecule of sugar (pentose) bonded to a nitrogen-containing organic ring compound. The nitrogen-containing compound is either a pyrimidine (cytosine, thymine, or uracil) or a purine (adenine or guanine). Nucleosides, derivatives and / or analogues can be used in many different industries and applications as in agrochemistry (herbicides, fungicides and insecticides), therapeutic drugs including a range of antiviral (HIV, hepatitis C virus and herpes simplex) and even antibiotics.

Legal Information

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Pseudouridine
    Cytidine
    3-methylcytidine methosulfate
    1-methyladenosine
    Uridine
    2-thiocytidine
    5-methylcytidine
    7-methylguanosine
    2′-O-methylcytidine
    Inosine
    Guanosine
    Ribothymidine
    See All (12)

standard

Product No.
Description
Pricing

M4254

5-Methylcytidine, ≥99%

M0627

7-Methylguanosine, ≥90%

U3750

Uridine, ≥99%

C4654

Cytidine, BioReagent, suitable for cell culture, powder, ≥99%

I4125

Inosine, ≥99% (HPLC)

G6752

Guanosine, ≥98%

47310-U

Nucleosides Test Mix, in 1% sodium formate (varied conc.), analytical standard

PHR1138

Adenosine, Pharmaceutical Secondary Standard; Certified Reference Material

PHR1514

Disodium Guanylate, Pharmaceutical Secondary Standard; Certified Reference Material

PHR2244

Uridine, Pharmaceutical Secondary Standard; Certified Reference Material

PHR2411

Guanosine, Pharmaceutical Secondary Standard; Certified Reference Material

PHR2340

5′-Adenylic Acid, Pharmaceutical Secondary Standard; Certified Reference Material

PHR3163

Zidovudine Related Compound D, pharmaceutical secondary standard

PHR3802

Cytidine, certified reference material, pharmaceutical secondary standard

Certificates of Analysis (COA)

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