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Key Documents

G005817

LC/MS (TOF) Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC

application for HPLC

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500 G
CN¥256.13
6 X 500 G
CN¥870.52

CN¥256.13

Please contact Customer Service for Availability
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500 G
CN¥256.13
6 X 500 G
CN¥870.52

About This Item

CN¥256.13

Please contact Customer Service for Availability

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile; (5:95, A:B, pH 7.0 with formic acid)
flow rate: 0.4 mL/min
column temp.: 35 °C
detector: ESI(+), full scan, m/z 200-800
injection: 0.5 μL
sample: each compound, 300 ng/mL in 1% formic acid acetonitrile:water, 75:25

suitability

application for HPLC

application(s)

clinical

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This Item
31307223506208290
Calcium chloride dihydrate Vetec™, reagent grade, 99%

V900269

Calcium chloride dihydrate

Calcium chloride dihydrate puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%

31307

Calcium chloride dihydrate

Calcium chloride dihydrate ACS reagent, ≥99%

223506

Calcium chloride dihydrate

Calcium chloride dihydrate ACS Reagent Grade.

208290

Calcium chloride dihydrate

assay

99%

assay

≥99%

assay

≥99%, 99.0-105.0% (ACS specification)

assay

≥99% (by assay)

grade

reagent grade

grade

ACS reagent, puriss. p.a.

grade

ACS reagent

grade

ACS reagent

mp

176 °C (dec.) (lit.)

mp

176 °C (dec.) (lit.)

mp

176 °C (dec.) (lit.)

mp

176 °C (dec.) (lit.)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 hPa ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

-

pH

5-8 (25 °C, 147 g/L)

pH

4.5-8.0 (25 °C, 5%)

pH

4.5-8.5 (25 °C, 5%)

pH

-

Analysis Note

The basic nature of these compounds makes them targets for HILIC chromatographic separation. HILIC mobile phases consist of a high composition of acetonitrile, which facilitates the direct analysis of precipitated plasma samples without the need for additional sample solvent exchange. In most cases, the high organic mobile phase also facilitates increased analyte response with ESI(+) MS detection. Separation was performed on an Ascentis Express HILIC Fused-Core HPLC column. High purity solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Amiodarone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®
    N-Desethylamiodarone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®
    Lidocaine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    (±)-Flecainide solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
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