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G005743

HPLC Analysis of Steroids on Ascentis® Express F5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 15 cm x 4.6 mm I.D., 2.7 μm particles (53591-U)
mobile phase: [A] water; [B] methanol; (50:50, A:B)
flow rate: 0.8 mL/min
pressure: 2393 psi (165 bar)
column temp.: 35 °C
detector: UV, 240 nm
injection: 5 μL
sample: 10 μg/mL of each in 90: 10, water: methanol

suitability

application for HPLC

application(s)

clinical

Analysis Note

Sample concentrations at 10 ug/mL in 90:10 water:methanol. Acetonitrile does not provide adequate selectivity on this phase. Mass spec was used for peak identification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Hydrocortisone
    Prednisolone
    Prednisone

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