G005664
LC/MS Analysis of Amino Acids on Ascentis® Express HILIC
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] 13 mM ammonium acetate in 10:90 (v/v) water: acetonitrile; [B] 13 mM ammonium acetate in water
gradient: held at 0% B for 1 min; 0 to 90% B in 19 min
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ESI(+)
injection: 10 μL
sample: 10 mg/L in Mobile Phase A
suitability
application for HPLC
application(s)
life science and biopharma
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analytical column
standard
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