G005624
HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express F5
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express F5, 5 cm x 3.0 mm I.D., 2.7 μm particles (53576-U)
mobile phase: [A] 20 mM potassium phosphate, dibasic, pH 6.2; [B] acetonitrile
gradient: 0% B for 1.5 min, 0-10% over 0.5 min; to 15% B in 1.5 min, 2 min re-equilibration at 0% B
flow rate: 1.5 mL/min
pressure: 4931 psi (340 bar)
column temp.: 35 °C
detector: UV, 210 nm
injection: 5 μL
sample: all vitamins in water, 10 μg/mL for nicotinic acid and folic acid, 12 μg/mL for thiamine hydrochloride and cyanocobalamin, (20 μg/mL for pyridoxine, 30 μg/mL for riboflavin, 40 μg/mL for niacineamide, 100 μg/mL for calcium pantothenate, 120 μg/mL for biotin)
suitability
application for HPLC
application(s)
vitamins, nutraceuticals, and natural products
Analysis Note
Legal Information
Analyte
analytical column
standard
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