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G004736

HPLC Analysis of Lysozyme on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles (Z777051)
mobile phase: 50 mM phosphate buffered saline, 200 mM Na2SO4, pH 7.0
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 214 nm
injection: 5 μL
sample: 1 mg/mL in 50 mM phosphate buffered saline

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

The positively-charged protein, lyzoyme (pI=11), exhibits excellent peak shape and recovery on SRT SEC-300 columns due to its highly-inert surface. Low protein recovery is usually attributed to 1) hydrophobic interactions with the stationary phase and 2) electrostatic interactions with the silica suface. SRT SEC-300 surface is hydrophilic and densely bonded to silica, therefore providing high mass recovery and good peak shape.

Legal Information

SRT is a registered trademark of Sepax Technologies

Analyte

Description
    Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

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