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Safety Information

L3790

Sigma-Aldrich

Lysozyme from chicken egg white

10 mg/mL

Synonym(s):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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1 KIT
CN¥1,004.32

CN¥1,004.32

Available to ship onApril 17, 2025Details

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1 KIT
CN¥1,004.32

About This Item

CAS Number:
12650-88-3
Enzyme Commission number:
3.2.1.17 (BRENDA, IUBMB)
MDL number:
MFCD00131557
UNSPSC Code:
12352204
NACRES:
NA.56

CN¥1,004.32

Available to ship onApril 17, 2025Details

biological source

chicken egg white

form

liquid

mol wt

single-chain 14.3 kDa

concentration

≥400000 units/mL
10 mg/mL

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

UniProt accession no.

P00698

application(s)

cell analysis

shipped in

wet ice

storage temp.

−20°C

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1 of 4

This Item
D4513D4527D5319
DNase I Amplification Grade

AMPD1

DNase I

Deoxyribonuclease I from bovine pancreas Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

D4513

Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I from bovine pancreas Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

D4527

Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I bovine recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein

D5319

Deoxyribonuclease I bovine

concentration

1 unit/μL

concentration

-

concentration

-

concentration

-

form

liquid

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous glycerol solution

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

General description

Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Suitable for the purification of both DNA and protein from bacteria.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.

Suitability

Tested for suitability with CelLytic™ B in protein extraction from bacteria.

Unit Definition

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Physical form

Ready to use solution:10 mg/mL lysozyme in 25 mM sodium acetate pH 4.5 with 50% glycerol.
Solution in 50% glycerol, 25 mM Sodium Acetate

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

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    Lot/Batch Number
    SLCQ4850
    SLCG2005
    SLBX4170
    SLBV6944
    SLBM3565V

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    Protocols

    Enzymatic Assay of Lysozyme (EC 3.2.1.17)

    This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

    Plasmid DNA Preparation

    Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

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