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Safety Information
10837059001
Roche
Lysozyme
from hen egg white
Synonym(s):
Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase, lysozyme
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0.5 MG
CN¥4,968.26
CN¥4,968.26
Available to ship onApril 17, 2025Details
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0.5 MG
CN¥4,968.26
About This Item
CN¥4,968.26
Available to ship onApril 17, 2025Details
Recommended Products
biological source
chicken egg white
Quality Level
Assay
≥95%
form
solid
specific activity
>23000 U/mg (Shugar units)
mol wt
14.4 kDa
packaging
pkg of 10 g
manufacturer/tradename
Roche
optimum pH
6.0-7.0
absorption
3.9 at 280 nm (10 mg enzyme/ml)
Related Categories
1 of 4
This Item | L2879 | L3790 | L4631 |
|---|---|---|---|
| specific activity >23000 U/mg (Shugar units) | specific activity ≥35,000 units/mg protein (E1%/282) | specific activity - | specific activity - |
| biological source chicken egg white | biological source chicken egg white | biological source chicken egg white | biological source chicken egg white |
| form solid | form lyophilized powder | form liquid | form powder |
| optimum pH 6.0-7.0 | optimum pH - | optimum pH - | optimum pH - |
| mol wt 14.4 kDa | mol wt ~14.3 kDa | mol wt single-chain 14.3 kDa | mol wt single-chain 14.3 kDa |
General description
Muramidase, Mucopeptide N-acetylmuramoyl-hydrolase
Application
Lysozyme has been used for:
- Cell wall degradation[1]
- Preparation of protoplasts[2]
- Bacteriolysis[3]
- Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics)
- Food and drinks (flavor enhancer)
- Sample preparation prior to isolation of nucleic acids[4]
Biochem/physiol Actions
Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.[7]
Unit Definition
One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C.
Preparation Note
Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Reconstitution
Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
动植物源性产品
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Kimberly M Davis et al.
Infection and immunity, 79(2), 562-570 (2010-11-03)
Bacterial pathogens that colonize mucosal surfaces have acquired resistance to antimicrobials that are abundant at these sites. One of the main antimicrobials present on mucosal surfaces is lysozyme, a muramidase that hydrolyzes the peptidoglycan backbone of bacteria. Cleavage of the
A new iron acquisition system in Bacteroidetes.
Manfredi P, et al.
Infection and Immunity (2014)
Hwi Hyun et al.
Frontiers in cellular and infection microbiology, 11, 676650-676650 (2021-09-17)
Recent studies have suggested the existence of a blood microbiome in the healthy host. However, changes in the blood microbiome upon bloodstream infection are not known. Here, we analyzed the dynamics of the blood microbiome in a porcine model of
Jan Hansen et al.
The Journal of chemical physics, 156(24), 244903-244903 (2022-07-02)
Intermolecular interactions in protein solutions, in general, contain many contributions. If short-range attractions dominate, the state diagram exhibits liquid-liquid phase separation (LLPS) that is metastable with respect to crystallization. In this case, the extended law of corresponding states (ELCS) suggests
Isolation of Heterocysts from Anabaena sp. PCC 7120
Maria Ermakova* and Yagut Allahverdiyeva
Bio-protocol, 5(8) (2015)
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