G004479
HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V
application for HPLC
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technique(s)
HPLC: suitable
test parameters
column: CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase: 15 mM ammonium formate in methanol
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 230 nm
injection: 5 μL
sample: 1 mg/mL in methanol
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Analysis Note
Sibutramine, one of a few drugs approved for long-term treatment of obesity, is a chiral compound. The (R) enantiomer has the desired effect of reducing body weight and food intake, but the (S) enantiomer has the opposite effect: A perfect example of why chiral HPLC is important to pharmaceutical and clinical analysts. Astec® CHIROBIOTIC® V successfully separates sibutramine enantiomers, using a simple, MS-friendly mobile phase.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
Analyte
Description
Sibutramine hydrochloride monohydrate, ≥98% (HPLC), solid
analytical column
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Description
Pricing
standard
Product No.
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Certificates of Analysis (COA)
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