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Key Documents

11024AST

Supelco

Astec® CHIROBIOTIC® V Chiral (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

V Chiral Stationary Phase Column

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10 μG
CN¥5,662.03

CN¥5,662.03

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10 μG
CN¥5,662.03

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

CN¥5,662.03

Please contact Customer Service for Availability

Product Name

Astec® CHIROBIOTIC® V Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L88

description

HPLC Column

product line

Astec®

packaging

pkg of 1 ea

manufacturer/tradename

Astec®

parameter

0-45 °C temperature
241 bar pressure (3500 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

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1 of 4

This Item
SRP6166H1404SRP6253
HGF human recombinant, expressed in CHO cells, ≥97% (SDS-PAGE), ≥97% (HPLC)

SRP6014

HGF human

HGF human recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

SRP6166

HGF human

Hepatocyte Growth Factor human HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

H1404

Hepatocyte Growth Factor human

EGF human recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE)

SRP6253

EGF human

biological source

human

biological source

human

biological source

human

biological source

human

recombinant

expressed in CHO cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in Baculovirus infected High-5 cells

recombinant

expressed in HEK 293 cells

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

assay

≥97% (HPLC), ≥97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

mol wt

80.017 kDa

mol wt

70 kDa (single chain, glycosylated)

mol wt

~80 kDa

mol wt

calculated mol wt 6.3 kDa, observed mol wt 6.3 kDa by SDS-PAGE (DTT-reduced.)

form

lyophilized

form

lyophilized

form

lyophilized powder

form

lyophilized powder

General description

Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC® V in the polar ionic mode. The CHIROBIOTIC® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.

  • Bonded phase: Vancomycin
  • Operating pH range: 3.5 - 7.0
  • Particle diameter: 5, 10 or 16 μm
  • Pore size: 100 Å (CHIROBIOTIC® V) or 200 Å (CHIROBIOTIC® V2)

Application

Astec® CHIROBIOTIC® V Chiral HPLC column may be used for the separation of different types of racemic compounds (ß-blockers, ß-agonists, benzodiazipines, non-steroidal anti-inflammatory drugs, barbiturates, free and derivatized amino acids in packed column subcritical fluid chromatography (pSFC).[1] It may also be used for the separation of citalopram enantiomers and its two N-demethylated metabolites and of alprenolol that is the internal standard, in human plasma using chiral liquid chromatography.[2] It may also be used in the chiral liquid chromatography analysis of ketoprofen and flurbiprofen.[3]

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Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Application

Product No.
Description
Pricing

G004402

HPLC Analysis of 3,5-tBu-MeOBIPHEP Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004404

HPLC Analysis of Promethazine Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004408

HPLC Analysis of Citalopram Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004471

HPLC Analysis of Mephenytoin Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004472

HPLC Analysis of Warfarin Enantiomers on Astec® CHIROBIOTIC® V (Method 1), application for HPLC

G004479

HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004505

HPLC Analysis of Methylphenidate Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004618

HPLC Analysis of Benzoin Methyl Ether Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004620

HPLC Analysis of Chloroquine Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004621

HPLC Analysis of Clenbuterol Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004622

HPLC Analysis of Devrinol (Napropamide) Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004623

HPLC Analysis of Bupivacaine Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004624

HPLC Analysis of Fenoprofen Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004625

HPLC Analysis of Fenoterol Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004626

HPLC Analysis of Flurbiprofen Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004627

HPLC Analysis of Hydroxychloroquine Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004628

HPLC Analysis of Ibuprofen Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004629

HPLC Analysis of Ketoprofen Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004630

HPLC Analysis of Labetalol Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004631

HPLC Analysis of Methylphenobarbital (Mephobarbital) Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004632

HPLC Analysis of Metoprolol Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004633

HPLC Analysis of Mianserin Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004634

HPLC Analysis of Pindolol Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004635

HPLC Analysis of Proglumide Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004636

HPLC Analysis of Sotalol Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004637

HPLC Analysis of Benoxaprofen Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004638

HPLC Analysis of Coumachlor Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004639

HPLC Analysis of Coumafuryl Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004640

HPLC Analysis of Isopromethazine Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004641

HPLC Analysis of Luciferin Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004642

HPLC Analysis of α-Methylbenzylamine Enantiomers (3,5-Dinitrobenzoyl Derivatives) on Astec® CHIROBIOTIC® V, application for HPLC

G004643

HPLC Analysis of 5-Methyl-5-Phenylhydantoin Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004644

HPLC Analysis of Naproxen Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004645

HPLC Analysis of Phensuximide Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004646

HPLC Analysis of Promethazine Sulfoxide Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004647

HPLC Analysis of Pyroglutethimide Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004648

HPLC Analysis of 3a,4,5,6-Tetrahydrosuccinimido[3,4-b]acenaphthen-10-one Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004649

HPLC Analysis of Warfarin Enantiomers on Astec® CHIROBIOTIC® V (Method 2), application for HPLC

G004651

HPLC Analysis of Fenoprofen Methyl Ester Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004652

HPLC Analysis of Indoprofen Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004653

HPLC Analysis of Thalidomide Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004654

HPLC Analysis of Nicardipine Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

G004349

HPLC Analysis of Warfarin Enantiomers on Astec® CHIROBIOTIC® V, application for HPLC

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    295024-06
    295024-05
    295024-04
    295024-03
    295024-02

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    Chiral packed column subcritical fluid chromatography on polysaccharide and macrocyclic antibiotic chiral stationary phases.
    Andrei M
    Journal of Chromatography A, 785 (1), 159-171 (1997)
    M Kosel et al.
    Journal of chromatography. B, Biomedical sciences and applications, 719(1-2), 234-238 (1998-12-30)
    A procedure using a chirobiotic V column is presented which allows separation of the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma. Citalopram, demethylcitalopram and didemethylcitalopram, as well as the internal
    F Péhourcq et al.
    Biomedical chromatography : BMC, 15(3), 217-222 (2001-06-08)
    Vancomycin is an amphoteric, glycopeptide, macrocyclic antibiotic. When attached to 5 microspherical silica gel, vancomycin proved to be an effective chromatographic chiral stationary phase that could be used in the reversed-phase mode. In this study, a bonded vancomycin chiral stationary
    Ana R Ribeiro et al.
    Chemosphere, 95, 589-596 (2013-11-05)
    Microbial degradation is the most important process to remove organic pollutants in Waste Water Treatment Plants. Regarding chiral compounds this process is normally enantioselective and needs the suitable analytical methodology to follow the removal of both enantiomers in an accurate
    Enantiomeric separation of mirtazapine and its metabolite in rat plasma by reverse polar ionic liquid chromatography using fluorescence and polarimetric detectors connected in series
    Rao, R. Nageswara, et al.
    Journal of Chromatography. B, Biomedical Sciences and Applications, 879 (21), 1911-1916 (2011)
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