G003761
HPLC Analysis of Amphetamines in Urine on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and Standard C18
application for SPE, application for HPLC
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¥434.44
Available to ship on2025年4月23日Details
Select a Size
About This Item
¥434.44
Available to ship on2025年4月23日Details
technique(s)
HPLC: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: human urine spiked amphetamine and methylamphetamine at with 2 mg/mL
SPE tube/cartridge: Discovery DSC-MCAX, 100 mg/3mL (52783-U)
condition: DSC-MCAX SPE tube: 1 mL methanol; 1 mL 50 mM ammonium acetate, pH 6.0; C-18 SPE tube: 1 mL methanol; 1 mL DI water
sample addition: 1 mL
washing: DSC-MCAX SPE tube: 1 mL 50 mM ammonium acetate, pH 6.0; 1 mL 1M acetic acid; 1mL methanol; C-18 SPE tube: 1 mL DI water; 1 mL 20% methanol
elution: DSC-MCAX SPE tube: 1 mL 5% ammonium hydroxide in methanol; C-18 SPE tube: 1 mL methanol
eluate post-treatment: evaporate to dryness with nitrogen at room temperature, reconstitute in mobile phase
column: Discovery HS F5, 15 cm x 4.6 mm I.D., 5 μm particles (567516-U)
mobile phase: [A] 10 mM ammonium acetate, pH 4.5: [B] methanol; (35:65, A:B)
flow rate: 2 mL/min
column temp.: 40 °C
detector: UV, 210 nm
injection: 10 μL
suitability
application for HPLC
application for SPE
application(s)
forensics and toxicology
1 of 4
This Item | GLYCOPRO | PROTSIL2 | G1041 |
|---|---|---|---|
| technique(s) protein staining: suitable | technique(s) - | technique(s) protein staining: suitable | technique(s) protein staining: suitable |
| usage sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels) | usage kit sufficient for 10 mini-gels (10 × 10 cm), kit sufficient for 5 gels or membranes (16.5 × 19 cm) | usage kit sufficient for 25 mini-gels (10 × 10 cm) | usage - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 20-25°C | storage temp. 2-8°C |
Application
1 mL human urine was spiked with 2 mg/mL amphetamine and methylamphetamine. The spiked sample was diluted 1:1 with 1:1 with 50 mM ammonium acetate, pH 6.0.
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