796-0477
HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase: [A] 20 mM Bis-Tris HCl, pH 7.0; [B] 20 mM Bis-Tris HCl, 0.5M NaCl, pH 7.0; 24 to 69% B in 20 min. (linear)
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μg each variant
suitability
application for HPLC
application(s)
life science and biopharma
Analysis Note
Legal Information
standard
Certificates of Analysis (COA)
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