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Safety Information

T8823

Sigma-Aldrich

Monoclonal Anti-T7 tag antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

Synonym(s):

T7 Tag antibody

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200 μG
CN¥5,540.21

CN¥5,540.21

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200 μG
CN¥5,540.21

About This Item

MDL number:
MFCD02689159
UNSPSC Code:
12352203
NACRES:
NA.56

CN¥5,540.21

Please contact Customer Service for Availability

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

T7 Tag

form

buffered aqueous solution

concentration

~2 mg/mL

technique(s)

western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase

isotype

IgM

shipped in

dry ice

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Monoclonal Anti-T7 tag antibody produced in mouse purified immunoglobulin, buffered aqueous solution

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B9183

Anti-HA-Biotin antibody, Mouse monoclonal

Anti-HA−TRITC antibody, Mouse monoclonal ~1 mg/mL, clone HA-7, purified from hybridoma cell culture

H9037

Anti-HA−TRITC antibody, Mouse monoclonal

Anti-V5 antibody, Mouse monoclonal clone V5-10, purified from hybridoma cell culture

V8012

Anti-V5 antibody, Mouse monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

storage temp.

−20°C

storage temp.

−20°C, 2-8°C

storage temp.

−20°C

storage temp.

−20°C

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.
The T7 tag is a 11 amino acid peptide,[1] encoded in the leader sequence of T7 bacteriophage gene 10.[2] This gene encodes a T7 major capsid protein.[3]

Immunogen

synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH

Application

Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.[4]

Physical form

Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

Antibody is suitable for ELISA and immunoblotting.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000235435
    0000235435
    0000129512
    0000111657
    092K4850

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    Design of high-throughput methods of protein production for structural biology
    Stevens RC
    Structure, 8(9), R177-R185 (2000)
    Prachi Singh et al.
    Medical oncology (Northwood, London, England), 34(8), 145-145 (2017-07-22)
    DNA methyltransferases make use of alternative splicing mechanism to generate various splice variants, but their role(s) in modulating DNA methylation patterns in the cells is unclear. Notably, DNMT3B alone contains nearly 40 different splice variants. In this study, we have
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