AB3790
Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG)
Chemicon®, from rabbit
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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Recommended Products
biological source
rabbit
antibody form
affinity purified immunoglobulin
clone
polyclonal
purified by
affinity chromatography
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Specificity
T7 (MASMTGGQQMG). Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Immunogen
Epitope: T7 (MASMTGGQQMG)
MASMTGGQQMG (T7) conjugated to KLH
Application
Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) detects level of T7 Epitope Tag & has been published & validated for use in IP, WB & IC.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western blot: 1:1,000-1:10,000 colorimetric; 1:1,000-1:30,000 ECL.
Immunocytochemistry: 1:100-1:400
ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.Immunofluorescence-suitable
Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use high quality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:100-1:400
ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.Immunofluorescence-suitable
Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use high quality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Liquid in PBS, pH 7.2 with 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lucas J McKinnon et al.
The Plant cell, 32(5), 1589-1609 (2020-03-15)
Protein folding is a complex cellular process often assisted by chaperones, but it can also be facilitated by interactions with lipids. Disulfide bond formation is a common mechanism to stabilize a protein. This can help maintain functionality amid changes in
Jayanth Kumar Palanichamy et al.
The Journal of clinical investigation, 126(4), 1495-1511 (2016-03-15)
Posttranscriptional control of gene expression is important for defining both normal and pathological cellular phenotypes. In vitro, RNA-binding proteins (RBPs) have recently been shown to play important roles in posttranscriptional regulation; however, the contribution of RBPs to cell specification is
Blanca Chinchilla et al.
Fish & shellfish immunology, 70, 343-350 (2017-09-09)
Non-virion (NV) protein is essential for an efficient replication increasing the pathogenicity of the Salmonid novirhabdovirus (formerly IHNV), Piscine novirhabdovirus (formerly VHSV), and Hirame novirhabdovirus (HIRV). The interferon system, apoptosis, and other immune-related genes are modulated by NV to finally
Hyung-Joon Kwon et al.
Nature communications, 7, 11686-11686 (2016-05-26)
NF-κB is a key transcription factor that dictates the outcome of diverse immune responses. How NF-κB is regulated by multiple activating receptors that are engaged during natural killer (NK)-target cell contact remains undefined. Here we show that sole engagement of
Dae Kwan Ko et al.
Nature plants, 9(8), 1333-1346 (2023-08-11)
Excessive accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress, which is an underlying cause of major crop losses and devastating human conditions. ER proteostasis surveillance is mediated by the conserved master regulator of the unfolded protein
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