T6674
Tumor Necrosis Factor-α human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym(s):
hTNF-α, TNF-α
About This Item
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Product Name
Tumor Necrosis Factor-α human, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥97% (SDS-PAGE)
form
powder
potency
0.02-0.3 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
~17.4 kDa
packaging
pkg of 5x10 μg
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... TNF(7124)
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Application
- to analyze the effects of cytokine TNFα-stressed human neuronal and glial (HNG) cells
- to investigate the molecular mechanisms of TNFα-mediated prolyl-4 hydroxylase α1 (P4Hα1) suppression
- to induce death-receptor-mediated apoptosis in HeLa cells.
Biochem/physiol Actions
Physical form
Analysis Note
Regulatory Information
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What is the package size of Product T6674, Tumor Necrosis Factor-α human?
It is a 10μg size-power form.
What are the units/mg of Product T6674, Tumor Necrosis Factor-α human?
1ug=1.1x10e5 units.
What is the solubility of this Product T6674, Tumor Necrosis Factor-α human?
This can be dissolved at 0.1 to 1.0 mg/ml in saline or buffer, stabilized with 0.1 to 1%BSA.
What is the EC50 of Product T6674, Tumor Necrosis Factor-α human?
Bioactivity(cell inhibition, L929 cells) is expressed by an EC50 of 0.02 to 0.05 ng/mL for T6674. EC50 (equivalent concentration) is the same as ED50 (equivalent dose, cytotoxicity, L929 cells) used for the bioactivity specification for T0157, Tumor Necrosis Factor-alpha, human recombinant expressed in Yeast.
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Protocols
WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.
XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
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