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Key Documents

T1819

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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0.2 ML
CN¥4,273.58
0.5 ML
CN¥8,903.44

CN¥4,273.58

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0.2 ML
CN¥4,273.58
0.5 ML
CN¥8,903.44

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

CN¥4,273.58

Please contact Customer Service for Availability
Request a Bulk Order

grade

BioPerformance Certified
Molecular Biology

Quality Level

200

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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technique(s)

indirect ELISA: 1:5,000

technique(s)

indirect ELISA: 1:20,000

technique(s)

indirect ELISA: 1:5,000

technique(s)

indirect ELISA: 1:10,000

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

isotype

IgG2b

isotype

IgG1

isotype

IgG2b

isotype

IgG1

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0.[1] It is applicable in electrophoretic studies and capillary electrochromatography.[2]

Application

Trizma® hydrochloride solution has been used:
  • as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii[3]
  • in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies[4]
  • for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies[5]

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCQ5982
    0000367041
    0000367040
    0000332498
    0000332500

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    A review of acellular immersion tests on bioactive glasses-influence of medium on ion release and apatite formation
    Nommeots-Nomm A, et al.
    International Journal of Applied Glass Science, 11(3) (11)
    Kaichun Zhu et al.
    Nature protocols, 1(6), 3088-3093 (2007-04-05)
    This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia coli cultures. Fermented bacteria are harvested using a hollow
    (Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
    Ferreira CMH, et al.
    Royal Society of Chemistry Advances, 5(39) (5)
    Production and characterisation of biodegradable PLA nanoparticles loaded with thymol to improve its antimicrobial effect
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    Parasites change their transcriptional systems in different developmental stages and in response to environmental changes. To investigate the molecular mechanisms that underlie transcriptional regulation, it is essential to identify the exact positions of the transcriptional start sites (TSSs) and characterize

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