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Safety Information

SHC012V

Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles

Contains a gene encoding TagRFP

Synonym(s):

MISSION®, MISSION® Control Transduction Particles

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Select a Size

25 μL
CN¥2,257.75
100 μL
CN¥4,582.18
200 μL
CN¥5,503.01

CN¥2,257.75

Available to ship onApril 27, 2025Details

Request a Bulk OrderRequest more information
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25 μL
CN¥2,257.75
100 μL
CN¥4,582.18
200 μL
CN¥5,503.01

About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

CN¥2,257.75

Available to ship onApril 27, 2025Details

Request a Bulk OrderRequest more information

Quality Level

200

product line

MISSION®

concentration

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

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P6623P1869P5872
Anti-Phosphoserine antibody, Mouse Monoclonal clone PSR-45, purified from hybridoma cell culture

P5747

Anti-Phosphoserine antibody, Mouse Monoclonal

Anti-Phosphothreonine antibody, Mouse monoclonal clone PTR-8, purified from hybridoma cell culture

P6623

Anti-Phosphothreonine antibody, Mouse monoclonal

Anti-Phosphotyrosine antibody, Mouse monoclonal clone pT-154, purified from hybridoma cell culture

P1869

Anti-Phosphotyrosine antibody, Mouse monoclonal

Anti-Phosphotyrosine antibody, Mouse monoclonal clone PT-66, purified from hybridoma cell culture

P5872

Anti-Phosphotyrosine antibody, Mouse monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

-

biological source

mouse

clone

PSR-45, monoclonal

clone

PTR-8, monoclonal

clone

pT-154, monoclonal

clone

PT-66, monoclonal

technique(s)

dot blot: suitable, microarray: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, western blot: 2.5-5.0 μg/mL using total rat brain extract.

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1 μg/mL, microarray: suitable, western blot: 5-10 μg/mL using A431 cell extracts

technique(s)

direct ELISA: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA, radioimmunoassay: suitable, western blot: 0.25-0.5 μg/mL using total cell extract of human platelets

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used to knockdown transient receptor potential vanilloid 1 receptor (TRPV1)-like receptors.[1] It has also been used to generate fluorescent cell lines.[2]
MISSION®pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used in generating fluorescent cell lines by transduction.[2] It has also been used in transfection of human dermal fibroblast (HDF), WI-38 and IMR-90 FBs to express tagRFP.[3]
To see more application data, protocols, vector maps visit sigma.com/shrna.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION TagRFP Control Transduction Particles are a positive control to monitor transduction efficiency. The MISSION TagRFP Control Transduction Particles are produced from the sequence-verified 8594 base pair lentivirus plasmid, pLKO.1-puro-CMV-TagRFP (SHC012).

This construct aids in interpretation of experimental design and results by providing a red fluorescent protein marker for monitoring success in transduction of your cells of interest.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagRFP is a trademark of Evrogen Co.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000309470
    02172028MN

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    High EMT signature score of invasive non-small cell lung cancer (NSCLC) cells correlates with NF kappa B driven colony-stimulating factor 2 (CSF2/GM-CSF) secretion by neighboring stromal fibroblasts
    Rudisch A, et al.
    PLoS ONE, 10(4), e0124283-e0124283 (2015)
    Activation of temperature-sensitive TRPV1-like receptors in ARC POMC neurons reduces food intake
    Jeong JH, et al.
    PLoS Biology, 16(4), e2004399-e2004399 (2018)
    A high-content image-based method for quantitatively studying context-dependent cell population dynamics.
    Garvey CM
    Scientific Reports, 6, 29752-29752 (2016)
    Albin Rudisch et al.
    PloS one, 10(4), e0124283-e0124283 (2015-04-29)
    We established co-cultures of invasive or non-invasive NSCLC cell lines and various types of fibroblasts (FBs) to more precisely characterize the molecular mechanism of tumor-stroma crosstalk in lung cancer. The HGF-MET-ERK1/2-CREB-axis was shown to contribute to the onset of the
    Jae Hoon Jeong et al.
    PLoS biology, 16(4), e2004399-e2004399 (2018-04-25)
    Proopiomelanocortin (POMC) neurons in the arcuate nucleus of the hypothalamus (ARC) respond to numerous hormonal and neural signals, resulting in changes in food intake. Here, we demonstrate that ARC POMC neurons express capsaicin-sensitive transient receptor potential vanilloid 1 receptor (TRPV1)-like

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