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P5747

Sigma-Aldrich

Anti-Phosphoserine antibody, Mouse Monoclonal

clone PSR-45, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine

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Select a Size

25 μL
CN¥2,257.75
100 μL
CN¥4,582.18
200 μL
CN¥5,503.01

CN¥2,257.75

Available to ship onApril 27, 2025Details

Request a Bulk Order
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25 μL
CN¥2,257.75
100 μL
CN¥4,582.18
200 μL
CN¥5,503.01

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

CN¥2,257.75

Available to ship onApril 27, 2025Details

Request a Bulk Order

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PSR-45, monoclonal

form

buffered aqueous solution

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

dot blot: suitable
indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA
microarray: suitable
western blot: 2.5-5.0 μg/mL using total rat brain extract.

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This Item
P6623P1869P5872
Anti-Phosphoserine antibody, Mouse Monoclonal clone PSR-45, purified from hybridoma cell culture

P5747

Anti-Phosphoserine antibody, Mouse Monoclonal

Anti-Phosphothreonine antibody, Mouse monoclonal clone PTR-8, purified from hybridoma cell culture

P6623

Anti-Phosphothreonine antibody, Mouse monoclonal

Anti-Phosphotyrosine antibody, Mouse monoclonal clone pT-154, purified from hybridoma cell culture

P1869

Anti-Phosphotyrosine antibody, Mouse monoclonal

Anti-Phosphotyrosine antibody, Mouse monoclonal clone PT-66, purified from hybridoma cell culture

P5872

Anti-Phosphotyrosine antibody, Mouse monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

-

biological source

mouse

clone

PSR-45, monoclonal

clone

PTR-8, monoclonal

clone

pT-154, monoclonal

clone

PT-66, monoclonal

technique(s)

dot blot: suitable, microarray: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, western blot: 2.5-5.0 μg/mL using total rat brain extract.

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1 μg/mL, microarray: suitable, western blot: 5-10 μg/mL using A431 cell extracts

technique(s)

direct ELISA: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA, radioimmunoassay: suitable, western blot: 0.25-0.5 μg/mL using total cell extract of human platelets

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Specificity

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.

Immunogen

phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
The antibody has been used for the detection of some phosphoserine containing proteins using immunoblotting and dot blotting.

Biochem/physiol Actions

Protein phosphorylation and dephosphorylation are basic signaling mechanisms that modify protein function in eukaryotic cells. Phosphorylation is a rare posttranslational event in normal tissues, however, the abundance of phosphorylated cellular proteins increases several folds following various activation processes. The main amino acids that are phosphorylated are tyrosine, serine, or threonine (pTyr/pSer/pThr), each having specific kinases that phosphorylate them and specific phosphatases that dephosphorylate them. Mono and polyclonal antibodies directed against phosphorylated residues were generated and found useful as analytical and preparative tools by enabling the identification, quantification, and immunoaffinity isolation of phosphorylated cellular proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pricing

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000411415
    0000411415
    0000401259
    0000373523
    0000359063

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    Find documentation for the products that you have recently purchased in the Document Library.

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    An integrated assessment of histopathological changes of the enteric neuromuscular compartment in experimental colitis
    Ippolito C, et al.
    Journal of Cellular and Molecular Medicine, 19(2), 485-500 (2015)
    Matteo Fornai et al.
    The Journal of pharmacology and experimental therapeutics, 356(2), 434-444 (2015-11-20)
    Parkinson's disease is frequently associated with gastrointestinal symptoms, mostly represented by constipation and defecatory dysfunctions. This study examined the impact of central dopaminergic denervation, induced by injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle, on distal colonic excitatory cholinergic
    Mechanisms of specificity in protein phosphorylation
    Ubersax JA and Ferrell Jr JE
    Nature Reviews in Molecular and Cell Biology, 8(7), 530-530 (2007)
    Ling Lai et al.
    Journal of pineal research, 45(4), 476-488 (2008-08-19)
    Melatonin, via its MT1 receptor, but not the MT2 receptor, can modulate the transcriptional activity of various nuclear receptors - estrogen receptor alpha (ERalpha) and retinoic acid receptor alpha (RARalpha), but not ERbeta- in MCF-7, T47D, and ZR-75-1 human breast
    Changes in expression of VE-cadherin and MMPs in endothelial cells: Implications for angiogenesis
    Kiran MS, et al.
    Vascular Cell, 3(1), 6-6 (2011)
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