Skip to Content
Merck
CN
All Photos(3)

Documents

Safety Information

SAB5500001

Sigma-Aldrich

Anti-beta-Actin antibody, Rabbit monoclonal

clone SP124, recombinant, expressed in proprietary host, affinity isolated antibody

Sign Into View Organizational & Contract Pricing

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

recombinant

expressed in proprietary host

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

SP124, monoclonal

species reactivity

human (tested)

species reactivity (predicted by homology)

chicken, mouse, rat, bovine, rabbit, pig, dog, hamster, frog

technique(s)

flow cytometry: 1:100
immunoblotting: 1:200
immunohistochemistry: 1:200

isotype

IgG

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

Related Categories

General description

ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1.
Beta-actin is a nonmuscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Immunogen

Synthetic peptide near the N-terminus of human beta-Actin protein.

Application

Anti-Actin-Beta antibody has been used in western blotting.

Biochem/physiol Actions

β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Soy isoflavones protect against H2O2-induced injury in human umbilical vein endothelial cells
Jin L, et al.
Molecular Medicine Reports, 12, 4476-4482 (2015)
DEK: A novel early screening and prognostic marker for breast cancer
Ying G and Wu Y
Molecular Medicine Reports, 12(5), 7491-7495 (2015)
Dan Li et al.
Chemico-biological interactions, 304, 124-130 (2019-03-09)
4-Hydroxynonenal (HNE), an end-product of lipid peroxidation generated in response to oxidative stress, has been implicated in the pathophysiology of chronic liver diseases. HNE is very reactive that forms Michael adducts with nucleophilic sites in DNA, lipids and proteins. At
Nicotinic ?7 receptor inhibits the acylation stimulating protein?induced production of monocyte chemoattractant protein?1 and keratinocyte?derived chemokine in adipocytes by modulating the p38 kinase and nuclear factor??B signaling pathways
Jiao ZY, et al.
Molecular Medicine Reports, 14(4), 2959-2966 (2016)
Guo Ying et al.
Molecular medicine reports, 12(5), 7491-7495 (2015-10-16)
The present study aimed to investigate the expression status and clinical implications of DEK in breast cancer, in order to contribute to developments in breast cancer management. DEK expression status was detected in 628 breast cancer specimens by western blot

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service