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Merck
CN

SAB5600204

Anti-β-Actin (ACTB) Antibody

rabbit monoclonal, RM112

Synonym(s):

ACTB, Actin cytoplastic 1, actin beta, beta-actin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Clone:
RM112, monoclonal
recombinant monoclonal
Species reactivity:
all
Application:
ChIP
ELISA
flow cytometry
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
Technique(s):
ChIP: 1:200
ELISA: suitable (assay dependent concentration)
flow cytometry: suitable (assay dependent concentration)
immunocytochemistry: 1:200
immunohistochemistry: 1:200
immunoprecipitation (IP): 1:200
western blot: 1:1000
Citations:
18
Uniprot accession no.:
P60709

Key Documents

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Product Name

Anti-beta-Actin antibody, Rabbit monoclonal, recombinant, expressed in HEK 293 cells, clone RM112, purified immunoglobulin

recombinant

expressed in HEK 293 cells

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM112, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

technique(s)

ChIP: 1:200
ELISA: suitable (assay dependent concentration)
flow cytometry: suitable (assay dependent concentration)
immunocytochemistry: 1:200
immunohistochemistry: 1:200
immunoprecipitation (IP): 1:200
western blot: 1:1000

isotype

IgG

NCBI accession no.

60

UniProt accession no.

P60709

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

Related Categories

Biochem/physiol Actions

This antibody reacts to beta-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A peptide corresponding to the N-terminus of beta-actin

Physical form

Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
Y0307491
T1003475
Q0601106

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Chung-Chih Tseng et al.
Scientific reports, 12(1), 10513-10513 (2022-06-23)
Oral squamous cell carcinoma (OSCC) is a common malignant tumor worldwide that is characterized by abnormal lesions or malignant hyperplasia of soft and hard tissues in the oral cavity. Previous research has found that HDAC6 may be a potential therapeutic
Shuhui Wu et al.
Clinical epigenetics, 16(1), 10-10 (2024-01-10)
Metastasis is the primary cause of recurrence and death in patients with papillary thyroid carcinoma (PTC). LncRNA ACTA2-AS1, a long non-coding RNA, acts as a tumor suppressor in multiple types of human malignancies, while the role of ACTA2-AS1 in PTC
Sphingosine Kinases at the Intersection of Pro-Inflammatory LPS and Anti-Inflammatory Endocannabinoid Signaling in BV2 Mouse Microglia Cells.
Standoli, et al.
International Journal of Molecular Sciences, 24 (2023)
Noemi Anna Pesce et al.
Journal of cellular and molecular medicine, 25(22), 10480-10493 (2021-10-09)
In retinopathy of prematurity (ROP), the abnormal retinal neovascularization is often accompanied by retinal neuronal dysfunction. Here, a rat model of oxygen-induced retinopathy (OIR), which mimics the ROP disease, was used to investigate changes in the expression of key mediators
Serena Colafrancesco et al.
Arthritis & rheumatology (Hoboken, N.J.), 74(4), 654-664 (2021-11-09)
Salivary gland epithelial cells (SGECs) are key cellular drivers in the pathogenesis of primary Sjögren's syndrome (SS); however, the mechanisms sustaining SGEC activation in primary SS remain unclear. We undertook this study to determine the role of autophagy in the
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