SAB4504337
Anti-phospho-ASK1 (pSer966) antibody produced in rabbit
affinity isolated antibody
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All Photos(4)
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 154 kDa
species reactivity
human, rat, mouse
concentration
~1 mg/mL
technique(s)
ELISA: 1:20000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer966)
Gene Information
human ... MAP3K5(4217)
General description
Apoptosis signal-regulating kinase 1 (ASK1) is a serine/threonine protein kinase. It belongs to the MAPKKK (mitogen-activated protein kinase) family. This gene is located on human chromosome 6q23.
Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as apoptosis signal-regulating kinase 1 (ASK1), is encoded by the gene mapped to human chromosome 6q22.33. The encoded protein belongs to the mitogen-activated protein kinase family. Porcine MAP3K5, which shares 97 % sequence identity with human MAP3K5, is characterized with a domain of unknown function (DUF4071) and a protein kinase domain.
Immunogen
The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.
Immunogen Range: 932-981
Immunogen Range: 932-981
Application
Anti-phospho-ASK1 (pSer966) antibody has been used in western blotting.
Anti-phospho-ASK1 (pSer966) antibody produced in rabbit has been used in immunoblot analysis.
Biochem/physiol Actions
Apoptosis signal-regulating kinase 1 (ASK1) is linked with apoptosis in cancer and heart tissues. It act as an upstream regulator of p38 and JNK (c-Jun N-terminal kinase) in cardiomyocytes death. Mutations in MAP3K5 gene result in β-thalassemia disease severity and hydroxyurea treatment efficacy.
Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), is one of the vital genes involved in the mitogen-activated protein kinase (MAPK) signaling cascade, which plays a crucial role in the cytoplasmic transduction of extracellular signals. Apoptosis signal-regulating kinase 1 (ASK1)-dependent mitochondrial apoptosis pathway, activated by 4SC-202, which is a novel class I HDAC inhibitor (HDACi), potentially stops hepatocellular carcinoma (HCC) cell proliferation. The encoded protein in response to oxidative stress, endoplasmic reticulum stress, infection and calcium influx plays a vital role in intracellular signaling pathways and biological functions including cell death, differentiation and inflammation. Genomic variation in MAP3K5 indicates β-thalassemia disease severity and hydroxyurea (HU) treatment efficiency. ASK1 plays a vital role in inhibition of the adipocytic differentiation process of the tumor cells and functions as an efficient therapeutic target for several human diseases including malignant fibrous histiocytomas.
Features and Benefits
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Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Frédéric Chibon et al.
Genes, chromosomes & cancer, 40(1), 32-37 (2004-03-23)
Malignant fibrous histiocytomas (MFHs) are aggressive tumors without any definable line of differentiation. We recently demonstrated that about 20% of them are characterized by high-level amplifications of the 12q14-q15 chromosome region, associated with either 1p32 or 6q23 band amplification. This
Lei Zhang et al.
Oxidative medicine and cellular longevity, 2018, 1023025-1023025 (2018-03-31)
The poor survival rate of mesenchymal stromal cells (MSC) transplanted into recipient lungs greatly limits their therapeutic efficacy for diseases like bronchopulmonary dysplasia (BPD). The aim of this study is to evaluate the effect of thioredoxin-1 (Trx-1) overexpression on improving
L Pu et al.
Genetics and molecular research : GMR, 15(3) (2016-08-16)
Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of
Meili Fu et al.
Biochemical and biophysical research communications, 471(2), 267-273 (2016-01-17)
The aim of the present study is to investigate the potential anti-hepatocellular carcinoma (HCC) cell activity by 4SC-202, a novel class I HDAC inhibitor (HDACi). The associated signaling mechanisms were also analyzed. We showed that 4SC-202 treatment induced potent cytotoxic
Je-Oh Lim et al.
International journal of molecular sciences, 22(18) (2021-09-29)
Titanium dioxide nanoparticles (TiO2NPs) are widely used in industrial and medicinal fields and in various consumer products, and their increasing use has led to an increase in the number of toxicity studies; however, studies investigating the underlying toxicity mechanism have
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